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Synonyms
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B-cell differentiation factor I, Eosinophil differentiation factor, TRF
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Species
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Human
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Accession
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P05113
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GeneID
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3567
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Source
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Escherichia coli.
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Molecular Weight
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重组人白介素-5蛋白Approximately 26.5 kDa, a disulfide-linked homodimeric protein containing two 116 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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MIPTEIPTSA LVKETLALLS THRTLLIANE TLRIPVPVHK NHQLCTEEIF QGIGTLESQT VQGGTVERLF KNLSLIKKYI DGQKKKCGEE RRRVNQFLDY LQEFLGVMNT EWIIES
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人白介素-5蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.1 ng/ml, corresponding to a specific activity of > 1.0 × 107 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 20mM PB, pH 7.5, 3 % trehalose, 0.1 % Tween-80.
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Endotoxin
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Less than 1 EU/µg of rHuIL-5 as determined by LAL method.
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Reconstitution
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重组人白介素-5蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人白介素-5蛋白
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SDS-PAGE
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Reference
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1. MV Milburn, AM Hassell, MH Lambert, et al. 1993. Nature, 363: 172-6.
2. JS Lee, HD Campbell, CA Kozak, et al. 1989. Somat Cell Mol Genet, 15: 143-52.
3. BH van Leeuwen, ME Martinson, GC Webb, et al. 1989. Blood, 73: 1142-8.
4. S Dubucquoi, P Desreumaux, A Janin, et al. 1994. J Exp Med, 179: 703-8.
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Background
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The IL-5 gene is located on chromosome 5 in humans, in close proximity to the genes encoding IL-3, IL-4, and granulocyte-macrophage colony-stimulating factor (GM-CSF), which are often co-expressed in TH2 cells. Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunroglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. IL-5 is a 115-amino acid (in human, 133 in the mouse) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL-3 and GM-CSF), this glycoprotein in its active form is a homodimer.
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