|
Synonyms
|
Interleukin-16 isoform 1, Lymphocyte chemoattractant factor, LCF
|
|
Species
|
Human
|
|
Accession
|
Q14005
|
|
GeneID
|
3603
|
|
Source
|
Escherichia coli.
|
|
Molecular Weight
|
重组人白细胞介素-16,121a.a。蛋白Approximately 12.4 kDa, a single non-glycosylated polypeptide chain containing 121 amino acids.
|
|
Quantity
|
2µg/10µg/1000µg
|
|
AA Sequence
|
SAASASAASD VSVESTAEAT VCTVTLEKMS AGLGFSLEGG KGSLHGDKPL TINRIFKGAA SEQSETVQPG DEILQLGGTA MQGLTRFEAW NIIKALPDGP VTIVIRRKSL QSKETTAAGD S
|
|
Purity
|
> 95 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
重组人白细胞介素-16,121a.a。蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral T Lymphocytes is in a concentration range of 50-500 ng/ml.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
|
|
Endotoxin
|
Less than 1 EU/µg of rHuIL-16, 121 a.a. as determined by LAL method.
|
|
Reconstitution
|
重组人白细胞介素-16,121a.a。蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重组人白细胞介素-16,121a.a。蛋白
|
|
|
SDS-PAGE
|
|
|
Reference
|
1. Baier M, Bannert N, Werner A, et al. 1997. Proc Natl Acad Sci U S A, 94: 5273-7.
2. Zhang Y, Center DM, Wu DM, et al. 1998. J Biol Chem, 273: 1144-9.
3. Cruikshank WW, Kornfeld H, Center DM. 2000. J Leukoc Biol, 67: 757-66.
4. Cruikshank WW, Center DM, Nisar N, et al. 1994. Proc Natl Acad Sci U S A, 91: 5109-13.
5. Mathy NL, Scheuer W, Lanzendorfer M, et al. 2000. Immunology, 100: 63-9.
|
|
Background
|
Human Interleukin-16 (IL-16) is also called lymphocyte chemoattractant factor (LCF) and it has three isoforms which are cleaved from pre-Interleukin-16. IL-16 is mostly secreted by lymphocytes, epithelial cells, eosinophils, and CD8+ T cells. It has many functions including induction of the IL-2Rα on T cells, suppression of human immunodeficiency virus (HIV) replication, inhibition of T-cell antigen receptor/CD3 mediated T-cell stimulation in mixed lymphocyte reactions and so on. It signals through CD4 receptor. Furthermore, recombinant human IL-16 (121 a.a.) contains 121 amino acid residues and it shares 85 % a.a. sequence identity with murine IL-16.
|
