重组人白细胞介素-10蛋白 rHuIL-10 上海康朗生物科技有限公司

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产品名称：重组人白细胞介素-10蛋白
产品型号：rHuIL-10
产品展商：KALANG
产品文档：无相关文档
发布时间：2018-08-19
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简单介绍

重组人白细胞介素-10蛋白与其它公司提供的重组蛋白不同，rHuIL-10蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人白细胞介素-10蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.

产品描述

重组人白细胞介素-10蛋白

Synonyms	CSIF
Species	Human
Accession	P22301
GeneID	3586
Source	Escherichia coli.
Molecular Weight	重组人白细胞介素-10蛋白Approximately 18.6 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids.
Quantity	2µg/10µg/1000µg
AA Sequence	SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	重组人白细胞介素-10蛋白Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin	Less than 1 EU/µg of rHuIL-10 as determined by LAL method.
Reconstitution	重组人白细胞介素-10蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组人白细胞介素-10蛋白
SDS-PAGE
Reference	1. Eskdale J, Kube D, Tesch H, et al. 1997. Immunogenetics. 46:120-8. 2. Tan JC, Braun S, Rong H, et al. 1995. J Biol Chem. 270:12906-11. 3. Pestka S, Krause CD, Sarkar D, et al. 2004. Annu Rev Immunol. 22:929-79.
Background	Interleukin-10 (IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the charter member of the IL-10 family of α-helical cytokines that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. IL-10 is secreted by many activated hematopoietic cell types as well as hepatic stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human IL-10 is active on mouse cells, mouse IL-10 does not act on human cells. IL-10 is a 178 amino acid molecule that contains two intrachain disulfide bridges and is expressed as a 36 kDa noncovalently associated homodimer. The IL-10 dimer binds to two IL-10 Rα/IL-10R1 chains, resulting in recruitment of two IL-10 Rβ/IL-10R2 chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3. IL-10Rβ does not bind IL-10 by itself but is required for signal transduction. IL-10 is a critical molecule in the control of viral infections and allergic and autoimmune inflammation. It promotes phagocytic uptake and Th2 responses but suppresses antigen presentation and Th1 proinflammatory responses.