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Species
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Human
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Accession
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P39059
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GeneID
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1306
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Source
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Escherichia coli.
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Molecular Weight
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Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 177 amino acids (C-terminal fragment of collagen XVIII).
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Quantity
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20µg/100µg/1000µg
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AA Sequence
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重组人内皮抑素蛋白NYEKPALHLA ALNMPFSGDI RADFQCFKQA RAAGLLSTYR AFLSSHLQDL STIVRKAERY SLPIVNLKGQ VLFNNWDSIF SGHGGQFNMH IPIYSFDGRD IMTDPSWPQK VIWHGSSPHG VRLVDNYCEA WRTADTAVTG LASPLSTGKI LDQKAYSCAN RLIVLCIENS FMTDARK
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Purity
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> 96 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The ED50 as determined by inhibiting the FGF basic-dependent proliferation of HUVEC migration is less than 2.0 µg/mL in the presence of Anti-Human Endostatin Polyclonal Antibody.
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Physical Appearance
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重组人内皮抑素蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rHuEndostatin as determined by LAL method.
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Reconstitution
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重组人内皮抑素蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人内皮抑素蛋白
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Reference
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1. HT Ren, H Hu, Y Li, et al. 2013. J Zhejiang Univ Sci B, 14: 224-30.
2. W Zhuo, Y Chen, X Song, et al. 2011. Front Med, 5: 336-40.
3. G HuangandL Chen. 2009. Cancer Biother Radiopharm, 24: 589-96.
4. DM Wei, Y Gao, XR Cao, et al. 2006. Biochem Biophys Res Commun, 345: 1398-404.
5. B Brankin, M Campbell, P Canning, et al. 2005. Exp Eye Res, 81: 22-31.
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Background
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Endostatin has been identified as a C-terminal fragment of Collagen type 18, a recently identified member of a family of collagen-like proteins referred to as multiplexin family. Endostatin specifically inhibits proliferation of endothelial cells although it does not affect the proliferation of EOMA cells. Endostatin also potently inhibits angiogenesis and tumor growth. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes. Recombinant Endosatin expressed in yeast causes G1 arrest of endothelial cells, and endostatin treatment results in apoptosis of HUVE and HMVE cells.
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