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Synonyms
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TNFSF9, CD137L
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Species
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Human
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Accession
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P41273
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GeneID
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8744
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Source
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Escherichia coli.
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Molecular Weight
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重组人4-1BB配体/ TNFSF9蛋白Approximately 19.4 kDa, a single non-glycosylated polypeptide chain containing 184 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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REGPELSPDD PAGLLDLRQG MFAQLVAQNV LLIDGPLSWY SDPGLAGVSL TGGLSYKEDT KELVVAKAGV YYVFFQLELR RVVAGEGSGS VSLALHLQPL RSAAGAAALA LTVDLPPASS EARNSAFGFQ GRLLHLSAGQ RLGVHLHTEA RARHAWQLTQ GATVLGLFRV TPEIPAGLPS PRSE
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Purity
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重组人4-1BB配体/ TNFSF9蛋白> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The ED50 as determined by stimulation of IL-8 production using human PBMC is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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重组人4-1BB配体/ TNFSF9蛋白Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/µg of rHu4-1BBL as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组人4-1BB配体/ TNFSF9蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人4-1BB配体/ TNFSF9蛋白
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SDS-PAGE
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Reference
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1. Salih HR, Kiener PA, Nussler V. 2002. Int J Clin Pharmacol Ther. 40:348-53.
2. Jang IK, Lee ZH, Kim YJ, et al. 1998. Biochem Biophys Res Commun. 242:613-20.
3. Michel J, Pauly S, Langstein J, et al. 1999. Immunology. 98:42-6.
4. Alderson MR, Smith CA, Tough TW, et al. 1994. Eur J Immunol. 24:2219-27.
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Background
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4-1BBL is a member of the tumor necrosis factor (TNF) receptor family. This receptor contributes to the clonal expansion, survival, and development of T cells. In addition, 4-1BBL expression is found on dendritic cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. CD137 has been shown to interact with TRAF2. The human 4-1BBL gene codes for a 254 amino acid type II transmembrane containing a 28 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 205 amino acid extracellular domain (ECD). The human 41BBL ECD shares 32 % and 35 % a.a. identity with murine and rat ECD.
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