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Synonyms
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TNFRSF9, CD137 Antigen, T-cell Antigen ILA
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Species
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Human
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Accession
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Q07011
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GeneID
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3604
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Source
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Escherichia coli.
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Molecular Weight
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重组人类4-1BB受体/ TNFRSF9蛋白Approximately 17.7 kDa, a single non-glycosylated polypeptide chain containing 166 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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ERTRSLQDPC SNCPAGTFCD NNRNQICSPC PPNSFSSAGG QRTCDICRQC KGVFRTRKEC SSTSNAECDC TPGFHCLGAG CSMCEQDCKQ GQELTKKGCK DCCFGTFNDQ KRGICRPWTN CSLDGKSVLV NGTKERDVVC GPSPADLSPG ASSVTPPAPA REPGHS
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人类4-1BB受体/ TNFRSF9蛋白Fully biologically active when compared to standard. The biological activity is determined by its inhibitory effect of IL-8 production using human peripheral blood mononuclear cells. About 90 % of inibition was seen using a concentration of 1 µg for both 4-1BB Ligand and 4-1BB Receptor.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM PB, pH 8.0, 150 mM NaCl.
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Endotoxin
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重组人类4-1BB受体/ TNFRSF9蛋白Less than 1 EU/μg of rHu4-1BB Receptor as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人类4-1BB受体/ TNFRSF9蛋白
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SDS-PAGE
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Reference
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1. Arch RHandThompson CB. 1998. Mol Cell Biol, 18: 558-65.
2. Barao I. 2012. Front Immunol, 3: 402.
3. Taraban VY, Rowley TF, O'Brien L, et al. 2002. Eur J Immunol, 32: 3617-27.
4. Marin V, Kakuda H, Dander E, et al. 2007. Exp Hematol, 35: 1388-97.
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Background
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4-1BB receptor, also named TNFRSF9 is a member of the TNF superfamily of receptors. It is mainly expressed on the surface of a variety of T cells, but also found in B cells, monocytes, and various transformed cell lines. 4-1BB receptor binds to 4-1BBL, and they co-stimulate activity for activated T cells. Signaling by 4-1BB Receptor has been implicated in the antigen-presentation process and generation of cytotoxic T cells. Crosslinking of 4-1BB Receptor enhances T cell proliferation, IL-2 secretion survival and cytolytic activity. Further, it can enhance immune activity to eliminate tumors in mice.
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