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重组人类肿瘤坏死因子-α/ TNFSF2,他的蛋白

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  • 产品名称:重组人类肿瘤坏死因子-α/ TNFSF2,他的蛋白
  • 产品型号:rHuTNF-α/TNFSF2, His
  • 产品展商:KALANG
  • 产品文档:无相关文档
  • 发布时间:2018-07-24
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简单介绍
重组人类肿瘤坏死因子-α/ TNFSF2,他的蛋白与其它公司提供的重组蛋白不同,rHuTNF-α/TNFSF2, His蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白,可表达出对细胞有毒性、易被蛋白酶降解的蛋白;并获得具有良好的可溶性,并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效,将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人类肿瘤坏死因子-α/ TNFSF2,他的蛋白(全长序列)产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述

重组人类肿瘤坏死因子-α/ TNFSF2,他的蛋白

Synonyms Tumor Necrosis Factor, TNFSF2, Cachectin, Differentiation-inducing factor , DIF, Necrosin, Cytotoxin
Species Human
Accession P01375
GeneID 7124
Source 重组人类肿瘤坏死因子-α/ TNFSF2,他的蛋白Escherichia coli.
Molecular Weight Approximately 18.3 kDa, a single, non-glycosylated polypeptide chain containing 164 amino acids with Met and 6 × His at N-terminus.
Quantity 10µg/50µg/1000µg
AA Sequence 重组人类肿瘤坏死因子-α/ TNFSF2,他的蛋白MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL
Purity > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107IU/mg in the presence of actinomycin D.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation 重组人类肿瘤坏死因子-α/ TNFSF2,他的蛋白Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.0.
Endotoxin Less than 1 EU/μg of rHuTNF-α/TNFSF2, His as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组人类肿瘤坏死因子-α/ TNFSF2,他的蛋白
SDS-PAGE
Reference 1. Davenport C, Kenny H, Ashley DT, et al. 2012. Eur J Clin Invest, 42: 1173-9.
2. Cavalcanti YV, Brelaz MC, Neves JK, et al. 2012. Pulm Med, 2012: 745483.
3. Sheng WS, Hu S, Ni HT, et al. 2005. J Leukoc Biol, 78: 1233-41.
4. Berthold-Losleben MandHimmerich H. 2008. Curr Neuropharmacol, 6: 193-202.
Background Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79 % homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.

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