重组人类胸腺基质**细胞生成素蛋白 rHuTSLP 上海康朗生物科技有限公司

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产品名称：重组人类胸腺基质**细胞生成素蛋白
产品型号：rHuTSLP
产品展商：KALANG
产品文档：无相关文档
发布时间：2018-09-11
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简单介绍

重组人类胸腺基质**细胞生成素蛋白与其它公司提供的重组蛋白不同，rHuTSLP蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人类胸腺基质**细胞生成素蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.

产品描述

重组人类胸腺基质**细胞生成素蛋白

Species	Human
Accession	Q969D9
GeneID	85480
Source	Escherichia coli.
Molecular Weight	重组人类胸腺基质细胞生成素蛋白**Approximately 15.1 kDa, a single non-glycosylated polypeptide chain containing 132 amino acids.
Quantity	2µg/10µg/1000µg
AA Sequence	MYDFTNCDFE KIKAAYLSTI SKDLITYMSG TKSTEFNNTV SCSNRPHCLT EIQSLTFNPT AGCASLAKEM FAMKTKAALA IWCPGYSETQ INATQAMKKR RKRKVTTNKC LEQVSQLQGL WRRFNRPLLK QQ
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	重组人类胸腺基质细胞生成素蛋白**Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using human IL-7Rα and human TSLP R co-transfected murine BaF3 pro-B cells is less than 0.3 ng/ml, corresponding to a specific activity of > 3.3 × 10⁶ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin	Less than 1 EU/μg of rHuTSLP as determined by LAL method.
Reconstitution	重组人类胸腺基质细胞生成素蛋白**We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组人类胸腺基质细胞生成素蛋白**
SDS-PAGE
Reference	1. Osborn MJ, Ryan PL, Kirchhof N, et al. 2004. Blood, 103: 843-51. 2. Liu YJ, Soumelis V, Watanabe N, et al. 2007. Annu Rev Immunol, 25: 193-219. 3. Lu N, Wang YH, Arima K, et al. 2009. J Exp Med, 206: 2111-9. 4. Ziegler SF. 2010. Curr Opin Immunol, 22: 795-9. 5. Takai T. 2012. Allergol Int, 61: 3-17.
Background	Thymic stromal lymphopoietin (TSLP) is a hemopoietic protein belonging to the cytokine family and is known to play an important role in the maturation of T cell populations through activation of antigen presenting cells. It is mainly expressed in a number of tissues including heart, liver and prostate. TSLP signals through a heterodimeric receptor complex composed of the thymic stromal lymphopoietin receptor and the IL-7R alpha chain. After binding STAT5 phosphorylation is induced resulting in the expression of downstream transcription factors. Like IL-7, TSLP induces phosphorylation of STAT3 and STAT5, but uses kinases other than the JAKs for activation. TSLP has the functions of enhancing the maturation of CD11c+ dendritic cells and inducing allergic inflammation by directly activating mast cells. It expression is linked to many disease states including asthma, inflammatory arthritis, atopic dermatitis, and eczema and other allergic states. But the factors inducing the activation of TSLP release are not clearly defined. Human TSLP shares approximately 43 % a.a. sequence identity with mouse TSLP.