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Synonyms
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TNFRSF17, BCM, CD269
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Species
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Human
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Accession
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Q02223
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GeneID
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608
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Source
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Escherichia coli.
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Molecular Weight
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重组人类B细胞成熟抗原/ TNFRSF17蛋白Approximately 5.4 kDa, a single non-glycosylated polypeptide chain containing 50 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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AGQCSQNEYF DSLLHACIPC QLRCSSNTPP LTCQRYCNAS VTNSVKGTNA
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人类B细胞成熟抗原/ TNFRSF17蛋白Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit APRIL-mediated proliferation of anti-IgM stimulated murine B cells is no less than 40 ng/ml, corresponding to a specific activity of > 2.5 × 104 IU/mg in the presence of 100 ng/ml human APRIL.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.
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Endotoxin
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重组人类B细胞成熟抗原/ TNFRSF17蛋白Less than 1 EU/μg of rHuBCMA as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人类B细胞成熟抗原/ TNFRSF17蛋白
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Reference
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1. Kim J, Gross JA, Dillon SR, et al. 2011. Autoimmunity, 44: 69-81.
2. Sun J, Feng J, Li Y, et al. 2006. Biochem Biophys Res Commun, 346: 1158-62.
3. Pelletier M, Thompson JS, Qian F, et al. 2003. J Biol Chem, 278: 33127-33.
4. Laabi Y, Gras MP, Brouet JC, et al. 1994. Nucleic Acids Res, 22: 1147-54.
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Background
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B-Cell Maturation Antigen (BCMA), encoded by the TNFRSF17 gene in humans. It is a member of the TNF receptor superfamily and a type III membrane protein containing one extracellular cysteine rich domain. It is expressed on mature B-cells and other B-cell lines. BCMA can binds to TNFSF13B/BLyS/BAFF and TNFSF13/APRIL, and has functions that promotes B-cell survival, plays a role in the regulation of humoral immunity, and activates NF-kappa-B and JNK BCMA.
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