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- 产品名称：重组人泛素偶联酶E2 I，His蛋白
- 产品型号：rHuUBE2I, His
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-08-14
- 在线询价
简单介绍
重组人泛素偶联酶E2 I，His蛋白与其它公司提供的重组蛋白不同，rHuUBE2I, His蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人泛素偶联酶E2 I，His蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组人泛素偶联酶E2 I，His蛋白
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Synonyms
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SUMO-protein Ligase, Ubiquitin Carrier Protein 9, Ubiquitin Carrier Protein I, Ubiquitin-conjugating Enzyme E2 I, Ubiquitin-protein Ligase I, p18
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Species
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Human
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Source
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Escherichia coli.
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Molecular Weight
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重组人泛素偶联酶E2 I，His蛋白Approximately 19.5 kDa, a single non-glycosylated polypeptide chain containing 158 amino acids (a.a.) of human UBE2I/UBC9 and 8 a.a. vector sequence including 6 × His tag at N-terminus.
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Quantity
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10µg/50µg/1000µg
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AA Sequence
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重组人泛素偶联酶E2 I，His蛋白MHHHHHHAMG TLNMSGIALS RLAQERKAWR KDHPFGFVAV PTKNPDGTMN LMNWECAIPG KKGTPWEGGL FKLRMLFKDD YPSSPPKCKF EPPLFHPNVY PSGTVCLSIL EEDKDWRPAI TIKQILLGIQ ELLNEPNIQD PAQAEAYTIY CQNRVEYEKR VRAQAKKFAP S
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Concentration
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See label.
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Data is not available.
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Physical Appearance
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重组人泛素偶联酶E2 I，His蛋白Sterile Colorless liquid.
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Formulation
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A 0.2 µm filtered concentrated solution in 50 mM HEPES, pH 7.6, with 125 mM NaCl, 10 % Glycerol, 1 mM DTT.
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Endotoxin
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Less than 1 EU/µg of rHuUBC9, His as determined by LAL method.
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Storage
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Store at -20 °C.
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重组人泛素偶联酶E2 I，His蛋白
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SDS-PAGE
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Reference
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1. Yukita A, Hosoya A, Ito Y, et al. 2012. Bone, 50: 1092-9.
2. Duan X, Trent JO, Ye H. 2009. Anticancer Agents Med Chem, 9: 51-4.
3. Niedenthal R. 2009. Methods Mol Biol, 497: 63-79.
4. Shang Q, Xu C, Zhang J, et al. 2009. Proteins, 76: 266-9.
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Background
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SUMO-Conjugating Enzyme UBC9 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2I gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The UBC9 accepts the ubiquitin-like proteins SUMO1-4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Additionally, It takes parts in catalysis the formation of poly-SUMO chains, sumoylation of FOXL2 and KAT5, and the segregation of nuclear architecture and chromosome.
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