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重组人巨噬细胞迁移抑制因子,蛋白

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  • 产品名称:重组人巨噬细胞迁移抑制因子,蛋白
  • 产品型号:rHuMIF, His
  • 产品展商:KALANG
  • 产品文档:无相关文档
  • 发布时间:2018-07-24
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简单介绍
重组人巨噬细胞迁移抑制因子,蛋白与其它公司提供的重组蛋白不同,rHuMIF, His蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白,可表达出对细胞有毒性、易被蛋白酶降解的蛋白;并获得具有良好的可溶性,并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效,将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人巨噬细胞迁移抑制因子,蛋白(全长序列)产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述

重组人巨噬细胞迁移抑制因子,蛋白

Synonyms GLF, L-dopachrome Isomerase, Phenylpyruvate Tautomerase
Species Human
Source Escherichia coli.
Molecular Weight 重组人巨噬细胞迁移抑制因子,蛋白Approximately 13.5 kDa, a single non-glycosylated polypeptide chain containing 117 amino acids, with 6 × His at the C-terminus.
Quantity 10µg/50µg/1000µg
AA Sequence MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFALEHHH HHH
Purity 重组人巨噬细胞迁移抑制因子,蛋白> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The specific activity is determined by binding rhCD74 in a functional ELISA.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/µg of rHuMIF, His as determined by LAL method.
Reconstitution 重组人巨噬细胞迁移抑制因子,蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组人巨噬细胞迁移抑制因子,蛋白
Reference 1. Edwards KM, Tomfohr LM, Mills PJ, et al. 2011. Sleep, 34: 161-3.
2. Delaloye J, De Bruin IJ, Darling KE, et al. 2012. Cytokine, 
3. Leu RW, Woodson PD, Whitley SB. 1977. J Reticuloendothel Soc, 22: 329-40.
4. Landolfo S. 1977. G Batteriol Virol Immunol, 70: 137-43.
5. Baugh JA, Chitnis S, Donnelly SC, et al. 2002. Genes Immun, 3: 170-6.
Background Migration Inhibitory Factor (MIF) is a secreted protein without a cleavable signal sequence and is secreted via a specialized, non-classical pathway. It is secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. MIF consists of two α-helices and six β-strands, four of which form a β-sheet. The two remaining β-strands interact with other MIF molecules, creating a trimer. Structure-function studies suggest MIF is bifunctional with segregated topology. The N- and C-termini mediate enzyme activity (in theory). Phenylpyruvate tautomerase activity (enol-to-keto) has been demonstrated and is dependent upon Pro at position 1. Amino acids 50-65(a.a.) have also been suggested to contain thiol-protein oxidoreductase activity. MIF has proinflammatory cytokine activity centered around (a.a.) 49 - 65. On fibroblasts, MIF induces, IL-1, IL-8 and MMP expression; on macrophages, MIF stimulates NO production and TNF-α release folllowing IFN-γ activation. MIF apparently acts through CD74 and CD44, likely in some form of trimeric interaction. Human MIF is active on mouse cells. Human MIF is 90%, 94%, 95%, and 90% aa identical to mouse, bovine, porcine and rat MIF, respectively.

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