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Synonyms
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Breast Cancer Estrogen-inducible Protein, PNR-2, Polypeptide P1.A (hP1.A), Protein pS2
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Species
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Human
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Accession
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P04155
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GeneID
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7031
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Source
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Escherichia coli.
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Molecular Weight
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重组人三叶因子1蛋白Approximately 13.2 kDa, a homodimer consisting of two 60 amino acid polypeptide chains, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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EAQTETCTVA PRERQNCGFP GVTPSQCANK GCCFDDTVRG VPWCFYPNTI DVPPEEECEF
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人三叶因子1蛋白Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/ml, corresponding to a specific activity of ＞ 100 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
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Endotoxin
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Less than 1 EU/µg of rHuTFF1 as determined by LAL method.
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Reconstitution
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重组人三叶因子1蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人三叶因子1蛋白
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SDS-PAGE
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Reference
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1. Seib T, Blin N, Hilgert K, et al. 1997. Genomics, 40: 200-2.
2. Kirikoshi HandKatoh M. 2002. Int J Oncol, 21: 655-9.
3. Marchbank T, Westley BR, May FE, et al. 1998. J Pathol, 185: 153-8.
4. Wang DG, Liu WH, Lundy FT, et al. 1998. J Pathol, 184: 408-13.
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Background
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Trefoil factor 1 belongs to the trefoil factor family that consists of three members named TFF1, TFF2 and TFF3. They are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. The TFFs are stable secretory proteins expressed highly in the gastrointestinal tract (gastric mucosa). TFF1 is an essential protein for normal differentiation of the antral and pyloric gastric mucosa and functions as a stabilizer of the mucous gel overlying the gastrointestinal mucosa that provides a physical barrier against various noxious agents. It is studied because of commonly expressed in tumors as well.
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