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Synonyms
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Spasmolysin, Spasmolytic polypeptide, SP
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Species
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Human
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Accession
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Q03403
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GeneID
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7032
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Source
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Escherichia coli.
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Molecular Weight
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重组人类三叶因子2蛋白Approximately 12.0 kDa, a single non-glycosylated polypeptide chain containing 106 amino acids, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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EKPSPCQCSR LSPHNRTNCG FPGITSDQCF DNGCCFDSSV TGVPWCFHPL PKQESDQCVM EVSDRRNCGY PGISPEECAS RKCCFSNFIF EVPWCFFPKS VEDCHY
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人类三叶因子2蛋白Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM PB, pH 7.4, 130 mM NaCl.
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Endotoxin
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Less than 1 EU/µg of rHuTFF2 as determined by LAL method.
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Reconstitution
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重组人类三叶因子2蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人类三叶因子2蛋白
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Reference
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1. Seib T, Blin N, Hilgert K, et al. 1997. Genomics, 40: 200-2.
2. Kirikoshi HandKatoh M. 2002. Int J Oncol, 21: 655-9.
3. Xue L, Aihara E, Podolsky DK, et al. 2010. Gut, 59: 1184-91.
4. Poulsen SS, Thulesen J, Christensen L, et al. 1999. Gut, 45: 516-22.
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Background
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Trefoil factor 2 encoded by the TFF2 gene in humans, belongs to the trefoil factor family that consists of three members named TFF1, TFF2 and TFF3. They are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. The TFFs are stable secretory proteins expressed highly in the gastrointestinal tract (gastric mucosa). TFF2 can inhibit gastrointestinal motility and gastric acid secretion. Additionally, it functions as a structural component of gastric mucus, possibly by stabilizing glycoproteins in the mucus gel through interactions with carbohydrate side chains.
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