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Synonyms
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FHF-2
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Species
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Human
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Accession
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Q92913
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GeneID
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2258
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Source
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Escherichia coli.
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Molecular Weight
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重组人成纤维细胞生长因子13蛋白Approximately 27.6 kDa, a single non-glycosylated polypeptide chain containing 245 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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MAAAIASSLI RQKRQARERE KSNACKCVSS PSKGKTSCDK NKLNVFSRVK LFGSKKRRRR RPEPQLKGIV TKLYSRQGYH LQLQADGTID GTKDEDSTYT LFNLIPVGLR VVAIQGVQTK LYLAMNSEGY LYTSELFTPE CKFKESVFEN YYVTYSSMIY RQQQSGRGWY LGLNKEGEIM KGNHVKKNKP AAHFLPKPLK VAMYKEPSLH DLTEFSRSGS GTPTKSRSVS GVLNGGKSMS HNEST
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人成纤维细胞生长因子13蛋白Test in process.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris, pH 8.5, 500 mM NaCl.
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Endotoxin
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Less than 1 EU/µg of rHuFGF-13 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组人成纤维细胞生长因子13蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人成纤维细胞生长因子13蛋白
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SDS-PAGE
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Reference
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1. Gerhard DS, Wagner L, Feingold EA, et al. 2004. Genome Res. 14:2121-7.
2. Wittmack EK, Rush AM, Craner MJ, et al. 2004. J Neurosci. 24:6765-75.
3. Smallwood PM, Munoz-Sanjuan I, Tong P, et al. 1996. Proc Natl Acad Sci U S A. 93:9850-7.
4. Olsen SK, Garbi M, Zampieri N, et al. 2003. J Biol Chem. 278:34226-36.
5. Hartung H, Feldman B, Lovec H, et al. 1997. Mech Dev. 64:31-9.
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Background
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Human FGF-13 encoded by the FGF13 gene, belongs to the FGF-11 subfamily which has four members FGF-11 to FGF-14. These four members were initially referred to as fibroblast growth factor homologous factors (FHFs) because they have high sequence identity with the other FGFs but did not activate FGF receptors (FGFRs) and were not generally considered members of the FGF family. FGF-13 plays a crucial role in neuron polarization and migration in the cerebral cortex. In murine FGF-13 RNA was detected in developing central nervous system in cells, and was also found throughout the peripheral nervous system.
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