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Synonyms
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TNFRSF6, CD95, Apo I, Fas Antigen
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Species
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Human
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Accession
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P25445
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GeneID
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355
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Source
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Escherichia coli.
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Molecular Weight
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重组人可溶性Fas受体/ TNFRSF6蛋白Approximately 17.6 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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RLSSKSVNAQ VTDINSKGLE LRKTVTTVET QNLEGLHHDG QFCHKPCPPG ERKARDCTVN GDEPDCVPCQ EGKEYTDKAH FSSKCRRCRL CDEGHGLEVE INCTRTQNTK CRCKPNFFCN STVCEHCDPC TKCEHGIIKE CTLTSNTKCK EEGSRSN
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人可溶性Fas受体/ TNFRSF6蛋白Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the cytotoxicity of Jurkat cells is between 10-15 µg/ml in the presence of 2 ng/ml of rHuFas Ligand.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rHusFasR/TNFRSF6 as determined by LAL method.
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Reconstitution
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重组人可溶性Fas受体/ TNFRSF6蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人可溶性Fas受体/ TNFRSF6蛋白
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SDS-PAGE
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Reference
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1. Zhang W, Ramdas L, Shen W, et al. 2003. Cancer Biol Ther, 2: 572-8.
2. Takubo T, Kumura T, Nishiki S, et al. 2000. Acta Haematol, 103: 165-7.
3. Lautrette C, Loum-Ribot E, Petit D, et al. 2006. Apoptosis, 11: 1195-204.
4. Kovacic N, Grcevic D, Katavic V, et al. 2010. Lab Invest, 90: 402-13.
5. Kaufmann T, Strasser A, Jost PJ. 2012. Cell Death Differ, 19: 42-50.
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Background
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Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL.
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