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Synonyms
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FAM19A2
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Species
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Human
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Accession
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Q8N3H0
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GeneID
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338811
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Source
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重组人类趋化因子样蛋白TAFA-2蛋白Escherichia coli.
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Molecular Weight
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Approximately 11.2 kDa, a single, non-glycosylated polypeptide chain containing 101 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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ANHHKAHHVK TGTCEVVALH RCCNKNKIEE RSQTVKCSCF PGQVAGTTRA APSCVDASIV EQKWWCHMQP CLEGEECKVL PDRKGWSCSS GNKVKTTRVT H
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组人类趋化因子样蛋白TAFA-2蛋白Fully biologically active when compared to standard. The biological activity is determined by its ability to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons. rHuTAFA-2, immobilized at 6-24 μg/mL on a 96 well plate, is able to significantly enhance neurite outgrowth.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rHuTAFA-2 as determined by LAL method.
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Reconstitution
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重组人类趋化因子样蛋白TAFA-2蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组人类趋化因子样蛋白TAFA-2蛋白
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SDS-PAGE
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Reference
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1. Tom Tang Y, Emtage P, Funk WD, et al. 2004. Genomics, 83: 727-34.
2. Sambo MH, Lewis I, Idris SH. 2010. Niger J Med, 19: 194-8.
3. Fischer C, Christ E, Korf HW, et al. 2012. Gen Comp Endocrinol, 177: 98-103.
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Background
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TAFA-2 also named FAM19A2, is a chemokinelike protein which is belonged to the FAM19/TAFA family. The family is a newly discovered and distantly related to MIP-1α. It contains 5 members and TAFA proteins are highly expressed in specific brain regions. Like other members of the TAFA family, with the exception of TAFA5, mature TAFA1 contains 10 regularly spaced cysteine residues. Human TAFA2 is 97 % a.a. identical to mouse TAFA2. The biological functions of TAFA family members remain to be determined, but they are postulated to act as brain-specific chemokines or neurokines that take parts in regulators of immune and nervous cells.
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