- 如果您对该产品感兴趣的话，可以
- 产品名称：重组人类印度刺猬Cys28IleIle蛋白
- 产品型号：rHuIHH C28II
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-07-24
- 在线询价
简单介绍
重组人类印度刺猬Cys28IleIle蛋白与其它公司提供的重组蛋白不同，rHuIHH C28II蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组人类印度刺猬Cys28IleIle蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组人类印度刺猬Cys28IleIle蛋白
|
Synonyms
|
HHG-2
|
|
Species
|
Human
|
|
Accession
|
Q14623
|
|
GeneID
|
3549
|
|
Source
|
Escherichia coli.
|
|
Molecular Weight
|
重组人类印度刺猬Cys28IleIle蛋白Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids.
|
|
Quantity
|
5µg/25µg/1000µg
|
|
AA Sequence
|
IIGPGRVVGS RRRPPRKLVP LAYKQFSPNV PEKTLGASGR YEGKIARSSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDRLNSLAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRNKYGLL ARLAVEAGFD WVYYESKAHV HCSVKSEHSA AAKTGG
|
|
Purity
|
> 96 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
重组人类印度刺猬Cys28IleIle蛋白Fully biologically active when compared to standard. The ED50 as determined by its ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells is 3.0-10 μg/ml.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2 µm filtered concentrated solution in 1 × PBS, pH 7.4.
|
|
Endotoxin
|
Less than 1 EU/µg of rHuIHH C28II as determined by LAL method.
|
|
Reconstitution
|
重组人类印度刺猬Cys28IleIle蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重组人类印度刺猬Cys28IleIle蛋白
|
|
|
Reference
|
1. Kumar S, Balczarek KA, Lai ZC. 1996. Genetics. 142:965-72.
2. Marigo V, Roberts DJ, Lee SM, et al. 1995. Genomics. 28:44-51.
3. Vortkamp A, Lee K, Lanske B, et al. 1996. Science. 273:613-22.
|
|
Background
|
IHH, encoded by the IHH gene in humans, belongs to the mammalian hedgehog family. It is expressed in ***** kidney and liver. The function of IHH is involved in chondrocyte differentiation, proliferation and maturation especially during endochondral ossification. It regulates its effects by feedback control of parathyroid hormone-related peptide (PTHrP). IHH is also involved in yolk sac vasculogenesis, playing an important role in differentiation of epiblast cells into endothelial and red blood cells. Human IHH shares 100 % amino acid sequence identity with murine.
|
