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- 产品名称：重组恒河猴猕猴白介素-5蛋白
- 产品型号：rRhIL-5
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-07-24
- 在线询价
简单介绍
重组恒河猴猕猴白介素-5蛋白与其它公司提供的重组蛋白不同，rRhIL-5蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组恒河猴猕猴白介素-5蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组恒河猴猕猴白介素-5蛋白
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Synonyms
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Eosinophil differentiation factor, TRF
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Species
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Rhesus Macaque
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Accession
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P48093
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GeneID
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710622
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Unigene
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Mmu.3362.
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Source
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重组恒河猴猕猴白介素-5蛋白Escherichia coli.
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Molecular Weight
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Approximately 26.1 kDa, a disulfide-linked homodimer of two 115 amino acid polypeptide chains.
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AA Sequence
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IPTEIPASAL VKETLALLST HRTLLIANET LRIPVPVHKN HQLCTEEIFQ GIGTLESQTV QGGTVERLFK NLSLIKKYIG GQKKKCGEER RRVNQFLDYL QEFLGVMNTE WIIES
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组恒河猴猕猴白介素-5蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 5 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, with 5 % Trehalose.
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Endotoxin
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重组恒河猴猕猴白介素-5蛋白Less than 0.1 EU/µg of rRhIL-5 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组恒河猴猕猴白介素-5蛋白
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Background
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Through binding to the IL-5 receptor, IL-5 stimulates B cell growth and increases immunroglobulin secretion. It is also a key mediator in eosinophil activation. Interleukin-5 has long been associated with the cause of several allergic diseases including allergic rhinitis and asthma. IL-5 is a 115-amino acid (in rhesus macaque, 133 in the murine) -long TH2 cytokine that is part of the hematopoietic family. Unlike other members of this cytokine family (namely IL-3 and GM-CSF), this glycoprotein in its active form is a homodimer.
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