重组恒河猴猕猴白细胞介素3蛋白 rRhIL-3 上海康朗生物科技有限公司

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产品名称：重组恒河猴猕猴白细胞介素3蛋白
产品型号：rRhIL-3
产品展商：KALANG
产品文档：无相关文档
发布时间：2018-09-19
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简单介绍

重组恒河猴猕猴白细胞介素3蛋白与其它公司提供的重组蛋白不同，rRhIL-3蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组恒河猴猕猴白细胞介素3蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.

产品描述

重组恒河猴猕猴白细胞介素3蛋白

Synonyms	Hematopoietic Growth Factor, MCGF, Multipotential Colony-stimulating Factor, P-cell-stimulating Factor
Species	Rhesus Macaque
Accession	P25140
GeneID	706946
Source	Escherichia coli.
Molecular Weight	重组恒河猴猕猴白细胞介素3蛋白Approximately 14.0 kDa, a single non-glycosylated polypeptide chain containing 124 amino acids.
Quantity	2µg/10µg/1000µg
AA Sequence	APMTQTTSLK TSWAKCSNMI DEIITHLNQP PLPSPDFNNL NEEDQTILVE KNLRRSNLEA FSKAVKSLQN ASAIESILKN LPPCLPMATA APTRPPIRIT NGDRNDFRRK LKFYLKTLEN EQAQ
Purity	> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity	重组恒河猴猕猴白细胞介素3蛋白Assay #1: Fully biologically active when compared to standard. The ED₅₀ as determined by the dose-dependent stimulation of the proliferation of murine NFS-60 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2 × 10⁵ IU/mg. Assay #2: The ED₅₀ as determined by a cell proliferation assay using human TF-1 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 10⁷ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	重组恒河猴猕猴白细胞介素3蛋白Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose.
Endotoxin	Less than 1 EU/µg of rRhIL-3 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组恒河猴猕猴白细胞介素3蛋白
Reference	1. Yang YC, Ciarletta AB, Temple PA, et al. 1986. Cell. 47:3-10. 2. Otsuka T, Miyajima A, Brown N, et al. 1988. J Immunol. 140:2288-95. 3. Dorssers L, Burger H, Bot F, et al. 1987. Gene. 55:115-24. 4. Feng Y, Klein BK, McWherter CA. 1996. J Mol Biol. 259:524-41.
Background	Interleukin-3 (IL-3) is an interleukin, a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. The rhesus macaque IL-3 reported to be a monomer, as it is known, contains 124 amino acids residues which is a single non-glycosylated polypeptide. Specifically, recombinant rhesus macaque, human and murine IL-3 share low homology.