- 如果您对该产品感兴趣的话，可以
- 产品名称：重组恒河猴猕猴白细胞介素-8 / CXCL8蛋白
- 产品型号：rRhIL-8/CXCL8
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-07-24
- 在线询价
简单介绍
重组恒河猴猕猴白细胞介素-8 / CXCL8蛋白与其它公司提供的重组蛋白不同，rRhIL-8/CXCL8蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组恒河猴猕猴白细胞介素-8 / CXCL8蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组恒河猴猕猴白细胞介素-8 / CXCL8蛋白
|
Species
|
Rhesus Macaque
|
|
Accession
|
P67813
|
|
GeneID
|
613028
|
|
Source
|
Escherichia coli.
|
|
Molecular Weight
|
重组恒河猴猕猴白细胞介素-8 / CXCL8蛋白Approximately 9.14kD containing 79 amino acid residues.
|
|
Quantity
|
5µg/25µg/1000µg
|
|
AA Sequence
|
AVLPRSAKEL RCECIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE PWVQRVVEKF VKRAENQNP
|
|
Purity
|
> 95 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
重组恒河猴猕猴白细胞介素-8 / CXCL8蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected murine BaF3 cells is in a concentration range of 0.5-5.0 ng/ml.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
|
|
Endotoxin
|
Less than 1 EU/μg of rRhIL-8/CXCL8 as determined by LAL method.
|
|
Reconstitution
|
重组恒河猴猕猴白细胞介素-8 / CXCL8蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重组恒河猴猕猴白细胞介素-8 / CXCL8蛋白
|
|
|
SDS-PAGE
|
|
|
Reference
|
1. Modi WS, Dean M, Seuanez HN, et al. 1990. Hum Genet. 84:185-7.
2. Wolff B, Burns AR, Middleton J, et al. 1998. J Exp Med. 188:1757-62.
3. Utgaard JO, Jahnsen FL, Bakka A, et al. 1998. J Exp Med. 188:1751-6.
4. Minnerly JC, Baganoff MP, Deppeler CL, et al. 1995. Inflammation. 19:313-31.
|
|
Background
|
Interleukin-8 (IL-8) is encoded by the IL8 gene and produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles. There are many receptors capable to bind IL-8, the most affinity to IL-8 are receptors CXCR1, and CXCR2. As a member of the CXC chemokine family, function of IL-8 is the induction of chemotaxis in its target cells, like neutrophil granulocytes, basophils, and T-cells. Rhesus macaque IL-8 has approximately 94 % amino acid sequence identity with human IL-8.
|
