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Synonyms
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TNFSF2, Cachectin, Differentiation-inducing Factor, DIF, Necrosin, Cytotoxin
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Species
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Rhesus Macaque
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Accession
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P48094
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GeneID
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715467
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Source
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Escherichia coli.
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Molecular Weight
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重组恒河猴猕猴肿瘤坏死因子-α/ TNFSF2蛋白Approximately 17.3 kDa, a single non-glycosylated polypeptide chain containing 157 amino acids.
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Quantity
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5µg/25µg/1000µg
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AA Sequence
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VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELTDNQLVV PSEGLYLIYS QVLFKGQGCP SNHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL GGVFQLEKGD RLSAEINLPD YLDFAESGQV YFGIIAL
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组恒河猴猕猴肿瘤坏死因子-α/ TNFSF2蛋白Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 107 IU/mg in the presence of actinomycin D.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 5 % trehalose.
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Endotoxin
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Less than 1 EU/μg of rRhTNF-α as determined by LAL method.
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Reconstitution
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重组恒河猴猕猴肿瘤坏死因子-α/ TNFSF2蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组恒河猴猕猴肿瘤坏死因子-α/ TNFSF2蛋白
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SDS-PAGE
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Reference
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1. Davenport C, Kenny H, Ashley DT, et al. 2012. Eur J Clin Invest, 42: 1173-9.
2. Cavalcanti YV, Brelaz MC, Neves JK, et al. 2012. Pulm Med, 2012: 745483.
3. Sheng WS, Hu S, Ni HT, et al. 2005. J Leukoc Biol, 78: 1233-41.
4. Berthold-Losleben MandHimmerich H. 2008. Curr Neuropharmacol, 6: 193-202.
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Background
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Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Rhesus macaque and human TNF-α show approximately 98 % homology at the amino acid level. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.
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