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Synonyms
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B-cell hybridoma growth factor, Interleukin HP-1.
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Species
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Murine
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Accession
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P08505
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GeneID
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16193
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Source
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Escherichia coli.
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Molecular Weight
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重组鼠白介素-6蛋白Approximately 21.7 kDa, a single non-glycosylated polypeptide chain containing 188 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组鼠白介素-6蛋白Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of IL-6-dependent murine 7TD1 cells is less than 0.02 ng/ml, corresponding to a specific activity of > 5 × 107 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/µg of rMuIL-6 as determined by LAL method.
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Reconstitution
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重组鼠白介素-6蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠白介素-6蛋白
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SDS-PAGE
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Reference
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1. Ferguson-Smith AC, Chen YF, Newman MS, et al. 1988. Genomics. 2:203-8.
2. van der Poll T, Keogh CV, Guirao X, et al. 1997. J Infect Dis. 176:439-44.
3. Ming JE, Cernetti C, Steinman RM, et al. 1989. J Mol Cell Immunol. 4:203-11; discussion 211-2.
4. Bastard JP, Jardel C, Delattre J, et al. 1999. Circulation. 99:2221-2.
5. Heinrich PC, Behrmann I, Muller-Newen G, et al. 1998. Biochem J. 334 ( Pt 2):297-314.
6. Van Snick J, Cayphas S, Szikora JP, et al. 1988. Eur J Immunol. 18:193-7.
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Background
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Interleukin-6 (IL-6) encoded by the IL-6 gene, acts as both a pro-inflammatory and anti-inflammatory cytokine. It is secreted by T cells and macrophages to stimulate immune response. It plays an essential role in the final differentiation of B-cells into Ig-secreting cells involved in lymphocyte and monocyte differentiation. It also induces myeloma and plasmacytoma growth and induces nerve cells differentiation acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. The mouse IL-6 is a single non-glycosylated polypeptide chain containing 187 amino acids and it signals through a cell-surface type I cytokine receptor complex consisting of the ligand-binding IL-6Rα chain (CD126), and the signal- transducing component gp130 (also called CD130). The mouse IL-6 shares 40 % and 85 % a.a. sequence identity with human and rat IL-6 and it is equally active on human and rat cells.
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