- 如果您对该产品感兴趣的话,可以
- 产品名称:重组小鼠生长分化因子7 /骨形态发生蛋白-12蛋白
- 产品型号:rMuGDF-7/BMP-12
- 产品展商:KALANG
- 产品文档:无相关文档
- 发布时间:2018-07-01
- 在线询价
简单介绍
重组小鼠生长分化因子7 /骨形态发生蛋白-12蛋白与其它公司提供的重组蛋白不同,rMuGDF-7/BMP-12蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白,可表达出对细胞有毒性、易被蛋白酶降解的蛋白;并获得具有良好的可溶性,并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效,将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组小鼠生长分化因子7 /骨形态发生蛋白-12蛋白(全长序列)产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组小鼠生长分化因子7 /骨形态发生蛋白-12蛋白
Species
|
Murine
|
Accession
|
P43029
|
GeneID
|
238057
|
Source
|
Escherichia coli.
|
Molecular Weight
|
重组小鼠生长分化因子7 /骨形态发生蛋白-12蛋白Approximately 29.8 kDa, a homodimeric protein consisting of two 146 amino acid non-glycosylated polypeptide chains.
|
AA Sequence
|
重组小鼠生长分化因子7 /骨形态发生蛋白-12蛋白TALAGTRGAQ GSGGGGGGGG GGGGGGGGGG GGAGRGHGRR GRSRCSRKSL HVDFKELGWD DWIIAPLDYE AYHCEGVCDF PLRSHLEPTN HAIIQTLLNS MAPDAAPASC CVPARLSPIS ILYIDAANNV VYKQYEDMVV EACGCR
|
Purity
|
> 95 % by SDS-PAGE and HPLC analyses.
|
Biological Activity
|
Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 0.5 μg/ml, corresponding to a specific activity of > 2000 IU/mg.
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
Formulation
|
重组小鼠生长分化因子7 /骨形态发生蛋白-12蛋白Lyophilized from a 0.2 µm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
|
Endotoxin
|
Less than 0.1 EU/µg of rMuGDF-7/BMP-12 as determined by LAL method.
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
Storage
|
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
重组小鼠生长分化因子7 /骨形态发���蛋白-12蛋白
|
|
Background
|
Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.
|