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- 产品名称:重组小鼠生长分化因子5 /骨形态发生蛋白-14蛋白
- 产品型号:rMuGDF-5/BMP-14
- 产品展商:KALANG
- 产品文档:无相关文档
- 发布时间:2018-08-04
- 在线询价
简单介绍
重组小鼠生长分化因子5 /骨形态发生蛋白-14蛋白与其它公司提供的重组蛋白不同,rMuGDF-5/BMP-14蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白,可表达出对细胞有毒性、易被蛋白酶降解的蛋白;并获得具有良好的可溶性,并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效,将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组小鼠生长分化因子5 /骨形态发生蛋白-14蛋白(全长序列)产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组小鼠生长分化因子5 /骨形态发生蛋白-14蛋白
Species
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Murine
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Accession
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P43027
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GeneID
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14563
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Source
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重组小鼠生长分化因子5 /骨形态发生蛋白-14蛋白Escherichia coli.
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Molecular Weight
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Approximately 27.2 kDa, a disulfide-linked homodimeric protein containing two 120 amino acids.
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AA Sequence
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APLANRQGKR PSKNLKARCS RKALHVNFKD MGWDDWIIAP LEYEAFHCEG LCEFPLRSHL EPTNHAVIQT LMNSMDPEST PPTCCVPTRL SPISILFIDS ANNVVYKQYE DMVVESCGCR
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Purity
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重组小鼠生长分化因子5 /骨形态发生蛋白-14蛋白> 96 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine ATDC5 cells is less than 1.0 μg/ml, corresponding to a specific activity of > 1000 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
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Endotoxin
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Less than 0.1 EU/µg of rMuGDF-5/BMP-14 as determined by LAL method.
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Reconstitution
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重组小鼠生长分化因子5 /骨形态发生蛋白-14蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 4 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组小鼠生长分化因子5 /骨形态发生蛋白-14蛋白
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Background
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Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.
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