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- 产品名称：重组鼠成纤维细胞生长因子17蛋白
- 产品型号：rMuFGF-17
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-08-19
- 在线询价
简单介绍
重组鼠成纤维细胞生长因子17蛋白与其它公司提供的重组蛋白不同，rMuFGF-17蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠成纤维细胞生长因子17蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组鼠成纤维细胞生长因子17蛋白
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Species
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Murine
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Source
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Escherichia coli.
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Molecular Weight
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Approximately 22.5 kDa, a single non-glycosylated polypeptide chain containing 194 amino acids.
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AA Sequence
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TQGENHPSPN FNQYVRDQGA MTDQLSRRQI REYQLYSRTS GKHVQVTGRR ISATAEDGNK FAKLIVETDT FGSRVRIKGA ESEKYICMNK RGKLIGKPSG KSKDCVFTEI VLENNYTAFQ NARHEGWFMA FTRQGRPRQA SRSRQNQREA HFIKRLYQGQ LPFPNHAERQ KQFEFVGSAP TRRTKRTRRP QSQT
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Purity
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> 98 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组鼠成纤维细胞生长因子17蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg in the presence of 10 μg/ml of heparin.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 20 mM Tris-HCl, pH 8.0, 700 mM NaCl, with 0.02 % Tween-20.
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Endotoxin
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Less than 0.1 EU/µg of rMuFGF-17 as determined by LAL method.
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Reconstitution
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重组鼠成纤维细胞生长因子17蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile PBS to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组鼠成纤维细胞生长因子17蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠成纤维细胞生长因子17蛋白
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Background
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FGF-17 is a member of the FGF superfamily of heparin-binding mitogenic molecules characterized by the presence of a core, 120 amino acid (aa) beta-trefoil structure. The mRNA of FGF-17 was found in midgestation of embryo and multiple ***** tissues, and is preferentially expressed in specific sites, such as embryonic brain, developing skeleton and arteries.
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