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Synonyms
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MIP-1-gamma, MRP-2, Small-inducible Cytokine A9
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Species
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Murine
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Accession
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P51670
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GeneID
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20308
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Source
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重组鼠巨噬细胞炎症蛋白-1 gamma / CCL9 / CCL10蛋白Escherichia coli.
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Molecular Weight
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Approximately 11.6 kDa, a single non-glycosylated polypeptide chain containing 101 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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QITHATETKE VQSSLKAQQG LEIEMFHMGF QDSSDCCLSY NSRIQCSRFI GYFPTSGGCT RPGIIFISKR GFQVCANPSD RRVQRCIERL EQNSQPRTYK Q
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组鼠巨噬细胞炎症蛋白-1 gamma / CCL9 / CCL10蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 0.1-10 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rMuMIP-1γ/CCL9/CCL10 as determined by LAL method.
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Reconstitution
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重组鼠巨噬细胞炎症蛋白-1 gamma / CCL9 / CCL10蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠巨噬细胞炎症蛋白-1 gamma / CCL9 / CCL10蛋白
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SDS-PAGE
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Reference
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1. Youn BS, Jang IK, Broxmeyer HE, et al. 1995. J Immunol. 155:2661-7.
2. Berahovich RD, Miao Z, Wang Y, et al. 2005. J Immunol. 174:7341-51.
3. Lean JM, Murphy C, Fuller K, et al. 2002. J Cell Biochem. 87:386-93.
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Background
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Murine CCL9/10 is belonging to the CC chemokine family. It is encoded by the gene CCL9. MRP-2 (CCL9/10) was isolated from a murine macrophage cell line, RAW 264.7. MRP-2 shows a 51 % sequence identity at the protein level to MRP-1 (C10) and 46% identity to MIP-1-alpha (CCL3). The functional receptor for CCL9/10 has been identified as CCR1. In contrast, other CCR1 ligands, CCL3/MIP-1alpha and CCL5/RANTES are considered to be primary CCR1 ligands in inflammatory responses. CCL9 can activate osteoclasts suggesting an important role for it in bone resorption. Murine CCL9/10 is 75 % amino acid identical to rat CCL9/10.
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