- 如果您对该产品感兴趣的话，可以
- 产品名称：重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白
- 产品型号：rMuEotaxin/CCL11
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-08-17
- 在线询价
简单介绍
重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白与其它公司提供的重组蛋白不同，rMuEotaxin/CCL11蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白
|
Synonyms
|
Small-inducible Cytokine A11
|
|
Species
|
Murine
|
|
Accession
|
P48298
|
|
GeneID
|
20292
|
|
Source
|
Escherichia coli.
|
|
Molecular Weight
|
重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 74 amino acids.
|
|
Quantity
|
5µg/20µg/1000µg
|
|
AA Sequence
|
HPGSIPTSCC FIMTSKKIPN TLLKSYKRIT NNRCTLKAIV FKTRLGKEIC ADPKKKWVQD ATKHLDQKLQ TPKP
|
|
Purity
|
> 96 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using purified eosinophils is in a concentration range of 100-1000 ng/ml.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
|
|
Endotoxin
|
Less than 1 EU/μg of rMuEotaxin/CCL11 as determined by LAL method.
|
|
Reconstitution
|
重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重组鼠嗜酸性粒细胞趋化因子/ CCL11蛋白
|
|
|
SDS-PAGE
|
|
|
Reference
|
1. Ponath PD, Qin S, Ringler DJ, et al. 1996. J Clin Invest. 97:604-12.
2. Jose PJ, Griffiths-Johnson DA, Collins PD, et al. 1994. J Exp Med. 179:881-7.
3. Garcia-Zepeda EA, Rothenberg ME, Ownbey RT, et al. 1996. Nat Med. 2:449-56.
4. Kitamura K, Singer WD, Star RA, et al. 1996. J Biol Chem. 271:7412-5.
|
|
Background
|
Murine CCL11 is belonging to the CC chemokine family. CCL11 was first purified from bronchoalveolar lavage fluid of guinea pigs. It was a strong and specific eosinophil chemoattractant in vitro. It can directly chemotactic for eosinophils, but not for monocytes or neutrophils. Murine CCL11 is approximately 63 % identical at the amino acid level to human CCL11. In addition, CCL11 also shows about 60 % amino acid sequence identity to human MCPs. CCR3 has been identified to be a specific CCL11 receptor.
|
