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重组小鼠干扰素-λ2/白细胞介素-28A蛋白

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  • 产品名称:重组小鼠干扰素-λ2/白细胞介素-28A蛋白
  • 产品型号:rMuIFN-λ2/IL-28A
  • 产品展商:KALANG
  • 产品文档:无相关文档
  • 发布时间:2018-09-04
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简单介绍
重组小鼠干扰素-λ2/白细胞介素-28A蛋白与其它公司提供的重组蛋白不同,rMuIFN-λ2/IL-28A蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白,可表达出对细胞有毒性、易被蛋白酶降解的蛋白;并获得具有良好的可溶性,并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效,将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组小鼠干扰素-λ2/白细胞介素-28A蛋白(全长序列)产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述

重组小鼠干扰素-λ2/白细胞介素-28A蛋白

Species Murine
Accession Q4VK74
GeneID 330496
Source Escherichia coli.
Molecular Weight 重组小鼠干扰素-λ2/白细胞介素-28A蛋白Approximately 19.7 kDa, a single non-glycosylated polypeptide chain containing 174 amino acids.
Quantity 5µg/20µg/1000µg
AA Sequence DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKDAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENMTDS ALATILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFRLL TRDLKCVASG DQCV
Purity > 95 % by SDS-PAGE and HPLC analyses.
Biological Activity 重组小鼠干扰素-λ2/白细胞介素-28A蛋白Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 105 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4, with 5 % trehalose.
Endotoxin Less than 0.1 EU/µg of rMuIFN-λ2/IL-28A as determined by LAL method.
Reconstitution 重组小鼠干扰素-λ2/白细胞介素-28A蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 ��C. Avoid repeated freeze/thaw cycles.
重组小鼠干扰素-λ2/白细胞介素-28A蛋白
SDS-PAGE

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