重组鼠巨噬细胞集落刺激因子蛋白 rMuM-CSF 上海康朗生物科技有限公司

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产品名称：重组鼠巨噬细胞集落刺激因子蛋白
产品型号：rMuM-CSF
产品展商：KALANG
产品文档：无相关文档
发布时间：2018-09-09
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简单介绍

重组鼠巨噬细胞集落刺激因子蛋白与其它公司提供的重组蛋白不同，rMuM-CSF蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠巨噬细胞集落刺激因子蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.

产品描述

重组鼠巨噬细胞集落刺激因子蛋白

Synonyms	CSF-1, MGI-IM
Species	Murine
Accession	Q3U4F9
Unigene	Mm.795.
Source	Escherichia coli.
Molecular Weight	重组鼠巨噬细胞集落刺激因子蛋白Approximately 52.0 kDa, a disulfide-linked homodimer consisting of two 230 amino acid polypeptide chains.
Quantity	2µg/10µg/1000µg
AA Sequence	KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE
Purity	重组鼠巨噬细胞集落刺激因子蛋白> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁵ IU/mg.
Physical Appearance	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, 500 mM NaCl, pH 7.4.
Endotoxin	重组鼠巨噬细胞集落刺激因子蛋白Less than 1 EU/μg of rMuM-CSF as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组鼠巨噬细胞集落刺激因子蛋白
Reference	1. Cosman D, Wignall J, Anderson D, et al. 1988. Behring Inst Mitt: 15-26. 2. Metcalf D, Willson T, Rossner M, et al. 1994. Growth Factors, 11: 145-52. 3. Hidaka T, Fujimura M, Nakashima A, et al. 2002. Jpn J Cancer Res, 93: 426-35. 4. Kubota Y, Takubo K, Shimizu T, et al. 2009. J Exp Med, 206: 1089-102.
Background	Macrophage Colony Stimulating Factor (M-CSF), also named CSF-1, is a hematopoietic growth factor that is involved in the proliferation, differentiation, and survival of monocytes, macrophages, and bone marrow progenitor cells. It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R. M-CSF is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer. Four transcript variants encoding three different isoforms have been reported for M-CSF gene. Although forms may vary, all of them contain the N-terminal 150 a.a. portion that is necessary and sufficient for interaction with the receptor. The first 229 a.a. of mature mouse M-CSF shares 87 %, 83 %, 82 % and 81 % sequence identity with corresponding regions of rat, dog, cow and human M-CSF, respectively. Human M-CSF is active in the mouse, but mouse M-CSF is reported to be species-specific.