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Synonyms
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Retinoic Acid-induced Differentiation Factor
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Species
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Murine
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Accession
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P12025
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GeneID
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17242
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Source
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Escherichia coli.
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Molecular Weight
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Approximately 13.2 kDa, a single non-glycosylated polypeptide chain containing 120 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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重组鼠midkine蛋白VAKKKEKVKK GSECSEWTWG PCTPSSKDCG MGFREGTCGA QTQRVHCKVP CNWKKEFGAD CKYKFESWGA CDGSTGTKAR QGTLKKARYN AQCQETIRVT KPCTSKTKSK TKAKKGKGKD
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Purity
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> 96 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组鼠midkine蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 10-100 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH7.4.
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Endotoxin
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Less than 1 EU/μg of rMuMidkine as determined by LAL method.
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Reconstitution
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重组鼠midkine蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at - 20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠midkine蛋白
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SDS-PAGE
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Reference
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1. Kaname T, Kuwano A, Murano I, et al. 1993. Genomics. 17:514-5.
2. Ikematsu S, Yano A, Aridome K, et al. 2000. Br J Cancer. 83:701-6.
3. Muramatsu T. 2002. J Biochem. 132:359-71.
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Background
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Midkine, also named MK, MK1, NEGF 2, is belonging to the neurotrophic and developmentally-regulated heparin-binding molecules family. It is encoded by the MDK gene. The Midkine protein includes five intrachain disulfide bonds which hold two domains and there are three antiparallel beta-sheets in each domain. A chondroitin sulfate proteoglycan, protein-tyrosine phosphatase zeta (PTPzeta), is a receptor for MK. MK promotes the growth, survival, and migration of various cells, and plays roles in neurogenesis and epithelial mesenchymal interactions during organogenesis. The predicted molecular weight is approximately 13.3 kDa, based on a mature peptide length of 118 amino acid residues in the mouse and 121 amino acid residues in the human. Across species, MK shows 87 % identity between the human and murine proteins.
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