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重组鼠头蛋白蛋白

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  • 产品名称:重组鼠头蛋白蛋白
  • 产品型号:rMuNoggin
  • 产品展商:KALANG
  • 产品文档:无相关文档
  • 发布时间:2018-07-24
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简单介绍
重组鼠头蛋白蛋白与其它公司提供的重组蛋白不同,rMuNoggin蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白,可表达出对细胞有毒性、易被蛋白酶降解的蛋白;并获得具有良好的可溶性,并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效,将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠头蛋白蛋白(全长序列)产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述

重组鼠头蛋白蛋白

Species Murine
Accession P97466
GeneID 18121
Source Escherichia coli.
Molecular Weight 重组鼠头蛋白蛋白Approximately 46.4 kDa, a disulfide-linked homodimer consisting of two 206 amino acid polypeptide chains.
Quantity 5µg/20µg/1000µg
AA Sequence MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGPAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC
Purity > 95 % by SDS-PAGE and HPLC analyses.
Biological Activity 重组鼠头蛋白蛋白Fully biologically active when compared to standard. The ED50 as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells is less than 2 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg in the presence of 5 ng/ml BMP-4.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 30 % acetonitrile, 0.1 % TFA.
Endotoxin Less than 1 EU/μg of rMuNoggin as determined by LAL method.
Reconstitution 重组鼠头蛋白蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HAc to a concentration less than 0.25 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组鼠头蛋白蛋白
Reference 1. Davis SWandCamper SA. 2007. Dev Biol, 305: 145-60.
2. Zhu W, Kim J, Cheng C, et al. 2006. Bone, 39: 61-71.
3. Oxley CD, Rashid R, Goudie DR, et al. 2008. Horm Res, 69: 221-6.
4. Cooper GM, Usas A, Olshanski A, et al. 2009. Plast Reconstr Surg, 123: 94S-103S.
5. Bayramov AV, Eroshkin FM, Martynova NY, et al. 2011. Development, 138: 5345-56.
Background Noggin encoded by the NOG gene, was first isolated from Xenopus, having the function of inducing secondary axis formation in frog embryos. It inhibits TGF-β family ligands and preventing them from binding to their corresponding receptors. Noggin was originally found as a BMP-4 antagonist, and then has been shown to modulate the activities of other BMPs (BMP-2, 7, 13 and 14). Additionally, it has pleiotropic effect, both in early development and later stages. The results of the mouse knockout of noggin suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. In recent report, proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have relation with the mutant of evolutionarily conserved amino acid residues of Noggin. Mature mouse Noggin shares 99 % and 83 % a.a. sequence identity with human and Xenopus Noggin, respectively.

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