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- 产品名称：重组鼠巨噬细胞炎症蛋白-2 / CXCL2蛋白
- 产品型号：rMuMIP-2/CXCL2
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-07-20
- 在线询价
简单介绍
重组鼠巨噬细胞炎症蛋白-2 / CXCL2蛋白与其它公司提供的重组蛋白不同，rMuMIP-2/CXCL2蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠巨噬细胞炎症蛋白-2 / CXCL2蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组鼠巨噬细胞炎症蛋白-2 / CXCL2蛋白
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Species
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Murine
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Accession
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P10889
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GeneID
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20310
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Source
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Escherichia coli.
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Molecular Weight
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重组鼠巨噬细胞炎症蛋白-2 / CXCL2蛋白Approximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KAN
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组鼠巨噬细胞炎症蛋白-2 / CXCL2蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 1.0-10 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
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Endotoxin
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Less than 1 EU/μg of rMuMIP-2/CXCL2 as determined by LAL method.
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Reconstitution
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重组鼠巨噬细胞炎症蛋白-2 / CXCL2蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠巨噬细胞炎症蛋白-2 / CXCL2蛋白
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Reference
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1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6.
2. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.
3. Wolpe SD, Sherry B, Juers D, et al. 1989. Proc Natl Acad Sci U S A. 86:612-6.
4. Iida N, Grotendorst GR. 1990. Mol Cell Biol. 10:5596-9.
5. Pelus LM, Fukuda S. 2006. Exp Hematol. 34:1010-20.
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Background
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Murine CXCL2, also named MIP-2, is belonging to the CXC chemokine family. It is encoded by the gene CXCL2. CXCL2 shares 90 % amino acid sequence with CXCL1/GROα. It is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. This chemokine is secreted from a murine macrophage cell line. The functional receptor for CXCL2 has been identified as CXCR2. CXCL2 is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. Similar to other GRO proteins, CXCL2 is potent neutrophil attractants and activators. In addition, it is also active toward basophils. The amino acid sequence of murine CXCL2 is 60 % identical to human CXCL2.
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