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Species
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Murine
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Accession
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Q6W5C0
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GeneID
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330122
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Source
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重组鼠树突状细胞炎症蛋白-1 / CXCL3蛋白Escherichia coli.
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Molecular Weight
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Approximately 7.9 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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重组鼠树突状细胞炎症蛋白-1 / CXCL3蛋白AVVASELRCQ CLNTLPRVDF ETIQSLTVTP PGPHCTQTEV IATLKDGQEV CLNPQGPRLQ IIIKKILKSG KSS
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.
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Physical Appearance
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重组鼠树突状细胞炎症蛋白-1 / CXCL3蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rMuDCIP-1/CXCL3 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠树突状细胞炎症蛋白-1 / CXCL3蛋白
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Reference
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1. Nolan KF, Strong V, Soler D, et al. 2004. J Immunol. 172:2201-9.
2. Modi WS, Yoshimura T. 1999. Mol Biol Evol. 16:180-93.
3. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83.
4. Smith DF, Galkina E, Ley K, et al. 2005. Am J Physiol Heart Circ Physiol. 289:H1976-84.
5. Wuyts A, Govaerts C, Struyf S, et al. 1999. Eur J Biochem. 260:421-9.
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Background
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CXCL3, also known as DCIP1 in murine, CINC2 in rat, and GROγ in humans, is belonging to the CXC chemokine family. It is encoded by the gene CXCL3 in mouse. The functional receptor for CXCL3 has been identified as CXCR2. Similar to other GRO proteins, CXCL3 is potent neutrophil attractants and activators. CXCL3 plays a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. The amino acid sequence of murine CXCL3 is 57 % identical to human CXCL3.
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