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- 产品名称：重组鼠血小板因子-4 / CXCL4蛋白
- 产品型号：rMuPF-4/CXCL4
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-06-19
- 在线询价
简单介绍
重组鼠血小板因子-4 / CXCL4蛋白与其它公司提供的重组蛋白不同，rMuPF-4/CXCL4蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠血小板因子-4 / CXCL4蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组鼠血小板因子-4 / CXCL4蛋白
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Species
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Murine
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Accession
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Q9Z126
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GeneID
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56744
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Source
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Escherichia coli.
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Molecular Weight
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重组鼠血小板因子-4 / CXCL4蛋白Approximately 8.2 kDa, a single non-glycosylated polypeptide chain containing 76 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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VTSAGPEESD GDLSCVCVKT ISSGIHLKHI TSLEVIKAGR HCAVPQLIAT LKNGRKICLD RQAPLYKKVI KKILES
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组鼠血小板因子-4 / CXCL4蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration of 10-100ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rMuPF-4/CXCL4 as determined by LAL method.
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Reconstitution
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重组鼠血小板因子-4 / CXCL4蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠血小板因子-4 / CXCL4蛋白
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Reference
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1. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.
2. Eisman R, Surrey S, Ramachandran B, et al. 1990. Blood. 76:336-44.
3. Lasagni L, Francalanci M, Annunziato F, et al. 2003. J Exp Med. 197:1537-49.
4. Warkentin TE. 2007. N Engl J Med. 356:891-3.
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Background
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CXCL4 (PF4) is a small cytokine belonging to the CXC chemokine family and it is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. CXCL4 interacts with a splice variant of the chemokine receptor CXCR3. Recombinant mouse CXCL4 contains 76 amino acids which is a single non-glycosylated polypeptide chain. Specifically, Human and mouse CXCL4 share about a 60 % identity.
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