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重组小鼠LPS诱导的CXC趋化因子/ CXCL5蛋白

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  • 产品名称:重组小鼠LPS诱导的CXC趋化因子/ CXCL5蛋白
  • 产品型号:rMuLIX/CXCL5
  • 产品展商:KALANG
  • 产品文档:无相关文档
  • 发布时间:2018-09-12
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简单介绍
重组小鼠LPS诱导的CXC趋化因子/ CXCL5蛋白与其它公司提供的重组蛋白不同,rMuLIX/CXCL5蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白,可表达出对细胞有毒性、易被蛋白酶降解的蛋白;并获得具有良好的可溶性,并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效,将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组小鼠LPS诱导的CXC趋化因子/ CXCL5蛋白(全长序列)产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述

重组小鼠LPS诱导的CXC趋化因子/ CXCL5蛋白

Synonyms Cytokine LIX, Small-inducible Cytokine B5
Species Murine
Accession P50228
GeneID 20311
Source 重组小鼠LPS诱导的CXC趋化因子/ CXCL5蛋白Escherichia coli.
Molecular Weight Approximately 9.8 kDa, a single, non-glycosylated polypeptide chain containing 92 amino acids.
Quantity 5µg/20µg/1000µg
AA Sequence APSSVIAATE LRCVCLTVTP KINPKLIANL EVIPAGPQCP TVEVIAKLKN QKEVCLDPEA PVIKKIIQKI LGSDKKKAKR NALAVERTAS VQ
Purity > 97 % by SDS-PAGE and HPLC analyses.
Biological Activity 重组小鼠LPS诱导的CXC趋化因子/ CXCL5蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood neutrophils is in a concentration of 10-100 ng/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Endotoxin Less than 1 EU/μg of rMuLIX/CXCL5 as determined by LAL method.
Reconstitution 重组小鼠LPS诱导的CXC趋化因子/ CXCL5蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组小鼠LPS诱导的CXC趋化因子/ CXCL5蛋白
SDS-PAGE
Reference 1. Chang MS, McNinch J, Basu R, et al. 1994. J Biol Chem. 269:25277-82.
2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.
3. Wuyts A, Haelens A, Proost P, et al. 1996. J Immunol. 157:1736-43.
4. Wuyts A, D'Haese A, Cremers V, et al. 1999. J Immunol. 163:6155-63.
Background CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78) and the gene for CXCL5 is encoded by the CXCL5 gene. It May participate in the recruitment of inflammatory cells by injured or infected tissue. CXCL5 is cleaved into the following 2 chains: GCP-2 (1-78) and GCP-2 (9-78) which attract neutrophils and are involved in neutrophil activation. Recombinant Mouse CXCL5 (92 a.a.) contains 92 amino acids and shares 57 % amino acid sequence identity with human CXCL5.

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