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Synonyms
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Gamma-interferon-induced Monokine, Small-inducible Cytokine B9
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Species
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Murine
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Accession
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P18340
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GeneID
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17329
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Source
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干扰素-γ/ CXCL9诱导的重组鼠单核因子蛋白Escherichia coli.
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Molecular Weight
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Approximately 12.2 kDa, a single non-glycosylated polypeptide chain containing 105 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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TLVIRNARCS CISTSRGTIH YKSLKDLKQF APSPNCNKTE IIATLKNGDQ TCLDPDSANV KKLMKEWEKK INQKKKQKRG KKHQKNMKNR KPKTPQSRRR SRKTT
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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干扰素-γ/ CXCL9诱导的重组鼠单核因子蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 0.1-1.0 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/µg of rMuMIG/CXCL9 as determined by LAL method.
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Reconstitution
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干扰素-γ/ CXCL9诱导的重组鼠单核因子蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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干扰素-γ/ CXCL9诱导的重组鼠单核因子蛋白
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SDS-PAGE
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Reference
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1. Lee HH, Farber JM. 1996. Cytogenet Cell Genet. 74:255-8.
2. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42.
3. Tensen CP, Flier J, Van Der Raaij-Helmer EM, et al. 1999. J Invest Dermatol. 112:716-22.
4. Adamska I, Roobol-Boza M, Lindahl M, et al. 1999. Eur J Biochem. 260:453-60.
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Background
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CXCL9 belongs to the CXC chemokine family and also known as Monokine induced by gamma interferon (MIG). It is a T-cell chemoattractant induced by IFN-γ. CXCL9 is closely related to two other CXC chemokines called CXCL10 and CXCL11, additionally they all elicit their chemotactic functions by interacting with the chemokine receptor CXCR3. It is a cytokine that affects the growth, movement, or activation state of cells that participate in immune and inflammatory response and chemotactic for activated T-cells. Recombinant murine CXCL9 contains 105 amino acids which is a single non-glycosylated polypeptide chain. Furthermore, The murine CXCL9 shares 75% and 88% a.a. sequence identity with human and rat CXCL9.
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