|
Synonyms
|
B-cell and Monocyte-activating Chemokine, Chemokine BRAK, Kidney-expressed Chemokine CXC, MIP-2G, Small-inducible Cytokine B14
|
|
Species
|
Murine
|
|
Accession
|
Q9WUQ5
|
|
GeneID
|
57266
|
|
Source
|
Escherichia coli.
|
|
Molecular Weight
|
重组鼠乳腺和肾表达的趋化因子/ CXCL14蛋白Approximately 9.4 kDa, a single non-glycosylated polypeptide chain containing 77amino acids.
|
|
Quantity
|
5µg/20µg/1000µg
|
|
AA Sequence
|
SKCKCSRKGP KIRYSDVKKL EMKPKYPHCE EKMVIVTTKS MSRYRGQEHC LHPKLQSTKR FIKWYNAWNE KRRVYEE
|
|
Purity
|
> 95 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml.
|
|
Physical Appearance
|
重组鼠乳腺和肾表达的趋化因子/ CXCL14蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 400 mM NaCl, pH 7.4, 5 % trehalose.
|
|
Endotoxin
|
Less than 1 EU/μg of rMuBRAK/CXCL14 as determined by LAL method.
|
|
Reconstitution
|
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20 °C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
重组鼠乳腺和肾表达的趋化因子/ CXCL14蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重组鼠乳腺和肾表达的趋化因子/ CXCL14蛋白
|
|
|
Reference
|
1. Hromas R, Broxmeyer HE, Kim C, et al. 1999. Biochem Biophys Res Commun. 255:703-6.
2. Starnes T, Rasila KK, Robertson MJ, et al. 2006. Exp Hematol. 34:1101-5.
3. Shurin GV, Ferris RL, Tourkova IL, et al. 2005. J Immunol. 174:5490-8.
4. Kurth I, Willimann K, Schaerli P, et al. 2001. J Exp Med. 194:855-61.
5. Hara T, Tanegashima K. 2012. J Biochem. 151:469-76.
6. Shellenberger TD, Wang M, Gujrati M, et al. 2004. Cancer Res. 64:8262-70.
|
|
Background
|
Chemokine (C-X-C motif) ligand 14 (CXCL14), also named BRAK, is a small cytokine belonging to the CXC chemokine family. It is constitutively expressed at the mRNA level in certain normal tissues and it possesses chemoattractive activity for activated macrophages, immature dendritic cells and natural killer cells. According to the relevant literature, CXCL14 is associated with tumor development. Moreover, recent evidence revealed that CXCL14 participates in glucose metabolism, feeding behaviour-associated neuronal circuits, and anti-microbial defense. Although CXCL14 receptors have not yet been identified, the intracellular activity of CXCL14 in breast cancer cells suggests that the CXCL14 receptor(s) and signal transduction pathway(s) may be different from those of conventional CXC-type chemokines. Recombinant murine CXCL14 contains 77 amino acid residues and it shares 97 % and 99 % a.a. sequence identity with human and rat CXCL14.
|
