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Synonyms
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ACT-2, MIP-1-beta, Protein H400, SIS-gamma, Small-inducible cytokine A4
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Species
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Murine
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Accession
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P14097
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GeneID
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20303
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Source
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Escherichia coli.
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Molecular Weight
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重组鼠巨噬细胞炎症蛋白-1 beta / CCL4蛋白Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 69 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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APMGSDPPTS CCFSYTSRQL HRSFVMDYYE TSSLCSKPAV VFLTKRGRQI CANPSEPWVT EYMSDLELN
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组鼠巨噬细胞炎症蛋白-1 beta / CCL4蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 20-100 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/µg of rMuMIP-1β/CCL4 as determined by LAL method.
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Reconstitution
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重组鼠巨噬细胞炎症蛋白-1 beta / CCL4蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠巨噬细胞炎症蛋白-1 beta / CCL4蛋白
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Reference
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1. Miyamoto MNaruo K, Seko C, et al. 1993. Mol Cell Biol. 13:4251-9.
2. Santos-Ocampo S, Colvin JS, Chellaiah A, et al. 1996. J Biol Chem. 271:1726-31.
3. Chellaiah A, Yuan W, Chellaiah M, et al. 1999. J Biol Chem. 274:34785-94.
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Background
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Chemokine (C-C motif) ligand 4, also known as Macrophage inflammatory protein-1β (MIP-1β) is a CC chemokine with specificity for CCR5 receptors and it is a major HIV-suppressive factor produced by CD8+ T cells. In addition, it is a monokine with inflammatory and chemokinetic properties. Recombinant CCL4 induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Furthermore, recombinant murine CCL4 contains 69 amino acids and it shares 77 % and 86 % a.a. sequence identity with human and rat CCL4. Both human and murine MIP-1α and MIP-1β are active on human and murine hematopoietic cells.
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