重组鼠白细胞介素-2蛋白 rMuIL-2 上海康朗生物科技有限公司

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产品名称：重组鼠白细胞介素-2蛋白
产品型号：rMuIL-2
产品展商：KALANG
产品文档：无相关文档
发布时间：2016-12-22
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简单介绍

重组鼠白细胞介素-2蛋白与其它公司提供的重组蛋白不同，rMuIL-2蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠白细胞介素-2蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.

产品描述

重组鼠白细胞介素-2蛋白

Synonyms	T-cell growth factor, TCGF, Aldesleukin
Species	Murine
Accession	P04351
GeneID	16183
Source	重组鼠白细胞介素-2蛋白Escherichia coli.
Molecular Weight	Approximately 17.2 kDa, a single non-glycosylated polypeptide chain containing 149 amino acids.
Quantity	5µg/20µg/1000µg
AA Sequence	重组鼠白细胞介素-2蛋白APTSSSTSSS TAEAQQQQQQ QQQQQQHLEQ LLMDLQELLS RMENYRNLKL PRMLTFKFYL PKQATELKDL QCLEDELGPL RHVLDLTQSK SFQLEDAENF ISNIRVTVVK LKGSDNTFEC QFDDESATVV DFLRRWIAFC QSIISTSPQ
Purity	> 95 % by SDS-PAGE and HPLC analyses.
Biological Activity	Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 10⁶ IU/mg.
Physical Appearance	重组鼠白细胞介素-2蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4.
Endotoxin	Less than 1 EU/µg of rMuIL-2 as determined by LAL method.
Reconstitution	We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage	This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组鼠白细胞介素-2蛋白
SDS-PAGE
Reference	1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79. 2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10. 3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51. 4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25. 5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6. 6. Matesanz, F., A. Alcina, and A. Pellicer. 1993. Immunogenetics, 38: 300-3.
Background	IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes; Mature mouse IL2 shares 56 % and 73 % amino acid sequence identity with human and rat IL2 respectively. Mouse and human IL2 exhibit cross-species activity. Mouse IL2 has a specific N-terminal region that contains a poly glutamine (contain 12 glutamines) stretch.