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重组鼠白细胞介素-3蛋白

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  • 产品名称:重组鼠白细胞介素-3蛋白
  • 产品型号:rMuIL-3
  • 产品展商:KALANG
  • 产品文档:无相关文档
  • 发布时间:2018-09-14
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简单介绍
重组鼠白细胞介素-3蛋白与其它公司提供的重组蛋白不同,rMuIL-3蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白,可表达出对细胞有毒性、易被蛋白酶降解的蛋白;并获得具有良好的可溶性,并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效,将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠白细胞介素-3蛋白(全长序列)产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述

重组鼠白细胞介素-3蛋白

Synonyms Hematopoietic growth factor, MCGF, Multipotential colony-stimulating factor, P-cell-stimulating factor.
Species Murine
Accession P01586
GeneID 16187
Source 重组鼠白细胞介素-3蛋白Escherichia coli.
Molecular Weight Approximately 14.8 kDa globular protein containing 134 amino acid residues.
Quantity 2µg/10µg/1000µg
AA Sequence DTHRLTRTLN CSSIVKEIIG KLPEPELKTD DEGPSLRNKS FRRVNLSKFV ESQGEVDPED RYVIKSNLQK LNCCLPTSAN DSALPGVFIR DLDDFRKKLR FYMVHLNDLE TVLTSRPPQP ASGSVSPNRG TVEC
Purity 重组鼠白细胞介素-3蛋白> 98 % by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by the dose-dependent stimulation of the proliferation of murine M-NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2 × 107 IU/mg.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4.
Endotoxin Less than 1 EU/µg of rMuIL-3 as determined by LAL method.
Reconstitution 重组鼠白细胞介素-3蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
重组鼠白细胞介素-3蛋白
SDS-PAGE
Reference 1. Yang YC, Ciarletta AB, Temple PA, et al. 1986. Cell. 47:3-10.
2. Otsuka T, Miyajima A, Brown N, et al. 1988. J Immunol. 140:2288-95.
3. Miyatake S, Yokota T, Lee F, et al. 1985. Proc Natl Acad Sci U S A. 82:316-20.
4. Dorssers L, Burger H, Bot F, et al. 1987. Gene. 55:115-24.
Background Interleukin-3 (IL-3) is a type of biological signal (cytokine) which is encoded by the IL-3 gene located on chromosome 5 and produced primarily by activated T cells beside human thymic epithelial cells, activated murine mast cells, murine keratinocytes and neurons/astrocytes. The protein acts in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. In addition, it exerts its biological activities through binding to interleukin-3 receptors included α and β subunits. The Mouse IL-3 is different from human IL-3 and contains 140 amino acids residues. Specifically, mouse and human IL-3 share low homology and have not cross species activity.

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