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Synonyms
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CSIF
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Species
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Murine
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Accession
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P18893
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GeneID
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16153
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Source
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Escherichia coli.
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Molecular Weight
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重组鼠白介素-10蛋白Approximately 18.7 kDa, a single non-glycosylated polypeptide chain containing 160 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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SRGQYSREDN NCTHFPVGQS HMLLELRTAF SQVKTFFQTK DQLDNILLTD SLMQDFKGYL GCQALSEMIQ FYLVEVMPQA EKHGPEIKEH LNSLGEKLKT LRMRLRRCHR FLPCENKSKA VEQVKSDFNK LQDQGVYKAM NEFDIFINCI EAYMMIKMKS
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组鼠白介素-10蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine MC/9-2 cells is less than 1 ng/ml, corresponding to a specific activity of > 1.0 × 106 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/µg of rMuIL-10 as determined by LAL method.
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Reconstitution
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重组鼠白介素-10蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10 mM HCl to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠白介素-10蛋白
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SDS-PAGE
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Reference
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1. Kim JM, Brannan CI, Copeland NG, et al. 1992. J Immunol. 148:3618-23.
2. Tan JC, Braun S, Rong H, et al. 1995. J Biol Chem. 270:12906-11.
3. Pestka S, Krause CD, Sarkar D, et al. 2004. Annu Rev Immunol. 22:929-79.
4. Vieira P, de Waal-Malefyt R, Dang MN, et al. 1991. Proc Natl Acad Sci U S A. 88:1172-6.
5. Hsu DH, de Waal Malefyt R, Fiorentino DF, et al. 1990. Science. 250:830-2.
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Background
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Interleukin-10 (IL-10) is encoded by the IL10 gene and expressed in monocytes and Type 2 T helper cells (Th2). The biological activities of IL-10 are mediated by the IL-10 receptor complex, which is composed of the ligand-binding IL-10 Rα/R1 and the accessory IL-10 Rβ/R2 subunits. IL-10 is capable of inhibiting the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by T helper cells. IL-10 is classified as a class 2 cytokine which consist of IL-10, IL-19, IL-20, IL-22, IL-24 and IL-26, several interferons and interferon-like molecules. Mature murine IL-10 shares 70 %-77 % amino acid sequence identity with human, bovine, porcine IL-10, but murine IL-10 does not act on human cells.
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