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- 产品名称：重组鼠白细胞介素-13蛋白
- 产品型号：rMuIL-13
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-06-21
- 在线询价
简单介绍
重组鼠白细胞介素-13蛋白与其它公司提供的重组蛋白不同，rMuIL-13蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠白细胞介素-13蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组鼠白细胞介素-13蛋白
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Species
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Murine
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Accession
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P20109
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GeneID
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16163
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Source
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Escherichia coli.
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Molecular Weight
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Approximately 12.3 kDa, a single non-glycosylated polypeptide chain containing 111 amino acids.
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Quantity
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重组鼠白细胞介素-13蛋白2µg/10µg/1000µg
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AA Sequence
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MPVPRSVSLP LTLKELIEEL SNITQDQTPL CNGSMVWSVD LAAGGFCVAL DSLTNISNCN AIYRTQRILH GLCNRKAPTT VSSLPDTKIE VAHFITKLLS YTKQLFRHGP F
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 4 ng/ml, corresponding to a specific activity of > 2.5 × 105 IU/mg.
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Physical Appearance
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重组鼠白细胞介素-13蛋白Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/µg of rMuIL-13 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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重组鼠白细胞介素-13蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠白细胞介素-13蛋白
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SDS-PAGE
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Reference
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1. Schmutz J, Martin J, Terry A, et al. 2004. Nature, 431: 268-74.
2. Wynn TA. 2003. Annu Rev Immunol, 21: 425-56.
3. Lakkis FG, Cruet EN, Nassar GM, et al. 1997. Biochem Biophys Res Commun, 235: 529-32.
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Background
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Murine Interleukin-13 (IL-13) is expressed by the IL13 gene located on the chromosome 11 and secreted by many cell types, especially T helper type 2 (Th2) cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 has been implicated as a key factor in asthma, allergy, atopy and inflammatory response, establishing the protein as a valuable therapeutic target. Human, mouse and rat IL-3 share low homology, but have cross species activity.
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