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Synonyms
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,HBGF-7
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Species
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Murine
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Accession
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P36363
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GeneID
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14178
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Source
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Escherichia coli.
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Molecular Weight
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重组鼠角质细胞生长因子1 / FGF-7蛋白Approximately 18.7 kDa, a single, non-glycosylated polypeptide chain containing 163 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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CNDMSPEQTA TSVNCSSPER HTRSYDYMEG GDIRVRRLFC RTQWYLRIDK RGKVKGTQEM KNSYNIMEIR TVAVGIVAIK GVESEYYLAM NKEGKLYAKK ECNEDCNFKE LILENHYNTY ASAKWTHSGG EMFVALNQKG IPVKGKKTKK EQKTAHFLPM AIT
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Purity
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> 96 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组鼠角质细胞生长因子1 / FGF-7蛋白Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 10 ng/ml, corresponding to a specific activity of > 1.0 × 105 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered solution in 20 mM PB, pH 8.0, 1 M NaCl.
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Endotoxin
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Less than 1 EU/µg of rMuKGF-1/FGF-7 as determined by LAL method.
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Reconstitution
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重组鼠角质细胞生长因子1 / FGF-7蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠角质细胞生长因子1 / FGF-7蛋白
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Reference
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1. Mattei MG, deLapeyriere O, Bresnick J, et al. 1995. Mamm Genome. 6:196-7.
2. Kelley MJ, Pech M, Seuanez HN, et al. 1992. Proc Natl Acad Sci U S A. 89:9287-91.
3. de Giorgi V, Sestini S, Massi D, et al. 2007. Dermatol Clin. 25:477-85, vii.
4. Eswarakumar VP, Lax I, Schlessinger J. 2005. Cytokine Growth Factor Rev. 16:139-49.
5. Belleudi F, Leone L, Nobili V, et al. 2007. Traffic. 8:1854-72.
6. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7.
7. Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700.
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Background
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Murine KGF-1 also known as Fibroblast growth factor 7 (FGF-7), is encoded by the FGF7 gene. KGF-1 only binds to the b splice form of the tyrosine kinase receptor, FGFR2b/KGFR. Affinity between KGF-1 and its receptor can be increased by heparin or heparan sulfate proteoglycan. FGF-10, also called keratinocyte growth factor 2 (KGF-2), shares 51 % amino acid sequence identity and similar function to KGF-1, but uses an additional receptor, FGFR2c. KGF-1 plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. KGF-1 actives on keratinocytes, and exhibits mitogenic activity for epidermal cells, but essentially no activity for fibroblasts. KGF-1 has species crossactive, murine KGF-1 shares 96 % amino acid sequence identity with human and rat.
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