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Species
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Murine
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Accession
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O35565
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GeneID
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14165
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Source
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Escherichia coli.
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Molecular Weight
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Approximately19.5 kDa, a single non-glycosylated polypeptide chain containing 173 amino acids.
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Quantity
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重组鼠角质细胞生长因子-2 / FGF-10蛋白5µg/25µg/1000µg
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AA Sequence
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QALGQDMVSQ EATNCSSSSS SFSSPSSAGR HVRSYNHLQG DVRWRRLFSF TKYFLTIEKN GKVSGTKNED CPYSVLEITS VEIGVVAVKA INSNYYLAMN KKGKLYGSKE FNNDCKLKER IEENGYNTYA SFNWQHNGRQ MYVALNGKGA PRRGQKTRRK NTSAHFLPMT IQT
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组鼠角质细胞生长因子-2 / FGF-10蛋白Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 106 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 2 × PBS, 600 mM NaCl, pH 7.4, 1 mM mercaptoethanol.
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Endotoxin
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Less than 1 EU/µg of rMuKGF-2/FGF-10 as determined by LAL method.
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Reconstitution
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重组鼠角质细胞生长因子-2 / FGF-10蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠角质细胞生长因子-2 / FGF-10蛋白
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Reference
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1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4.
2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404.
3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63.
4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5.
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Background
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Fibroblast growth factor 10 belongs to the fibroblast growth factor (FGF) family, which is involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may take parts in wound healing and is required for normal branching morphogenesis. Recombinant murine FGF-10 contains a 173 amino acids and it shares 94 % and 100 % a.a. sequence identity with human and rat FGF-10.
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