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- 产品名称：重组鼠单核细胞趋化蛋白-5 / CCL12蛋白
- 产品型号：rMuMCP-5/CCL12
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-07-20
- 在线询价
简单介绍
重组鼠单核细胞趋化蛋白-5 / CCL12蛋白与其它公司提供的重组蛋白不同，rMuMCP-5/CCL12蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠单核细胞趋化蛋白-5 / CCL12蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组鼠单核细胞趋化蛋白-5 / CCL12蛋白
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Synonyms
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MCP-1-related Chemokine, Small-inducible Cytokine A12
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Species
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Murine
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Accession
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Q62401
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GeneID
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20293
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Source
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重组鼠单核细胞趋化蛋白-5 / CCL12蛋白Escherichia coli.
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Molecular Weight
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Approximately 9.3 kDa, a single non-glycosylated polypeptide chain containing 82 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组鼠单核细胞趋化蛋白-5 / CCL12蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-50 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/μg of rMuMCP-5/CCL12 as determined by LAL method.
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Reconstitution
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重组鼠单核细胞趋化蛋白-5 / CCL12蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠单核细胞趋化蛋白-5 / CCL12蛋白
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Reference
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1. Sarafi MN, Garcia-Zepeda EA, MacLean JA, et al. 1997. J Exp Med. 185:99-109.
2. Jia GQ, Gonzalo JA, Lloyd C, et al. 1996. J Exp Med. 184:1939-51.
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Background
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Murine CCL12 is belonging to the CC chemokine family. It shows 66 % amino acid sequence identity to human MCP-1. CCL12 has been shown to be a potent chemoattractant for monocytes and lymphocytes but not neutrophils. At high concentrations, it will also chemoattract eosinophils. CCL12 can induce a calcium flux in human HEK-293 cells transfected with murine CCR2, but can not induce a calcium flux in HEK-293 cells transfected with CCR1, CCR3, or CCR5. That shows the functional receptor of CCL12 is CCR2.
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