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Synonyms
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Beta-chemokine Dxodus-3, CK beta-11, EBI1 Ligand Dhemokine, ELC Macrophage Inflammatory Protein 3 beta
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Species
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Murine
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Accession
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O70460
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GeneID
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24047
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Source
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Escherichia coli.
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Molecular Weight
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重组鼠巨噬细胞炎症蛋白-3 beta / CCL19蛋白Approximately 9.2 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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GANDAEDCCL SVTQRPIPGN IVKAFRYLLN EDGCRVPAVV FTTLRGYQLC APPDQPWVDR IIRRLKKSSA KNKGNSTRRS PVS
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组鼠巨噬细胞炎症蛋白-3 beta / CCL19蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human mature dendritic cells is in a concentration range of 10-100 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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重组鼠巨噬细胞炎症蛋白-3 beta / CCL19蛋白Less than 1 EU/μg of rMuMIP-3β/CCL19 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠巨噬细胞炎症蛋白-3 beta / CCL19蛋白
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Reference
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1. Milicevic NM, Miljkovic MD, Milicevic Z, et al. 2011. Histochem Cell Biol, 135: 593-601.
2. Guerin LR, Moldenhauer LM, Prins JR, et al. 2011. Biol Reprod, 85: 397-408.
3. Yoshida R, Imai T, Hieshima K, et al. 1997. J Biol Chem, 272: 13803-9.
4. Gibejova A, Mrazek F, Subrtova D, et al. 2003. Am J Respir Crit Care Med, 167: 1695-703.
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Background
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CCL19, also known as EBI1 Ligands chemokine (ELC) and macrophage inflammatory protein-3-beta, is encoded by the CCL19 gene which is located on chromosome 4 in mouse. The protein is expressed at high levels in thymus and lymph nodes, with intermediate levels in trachea and colon and low levels in stomach, lung and some other organs. CCL19 is signal through CCR7 and chemotaxis of T and B lymphocytes and myeloid progenitor cells.
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