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Synonyms
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6Ckine, Beta-chemokine Exodus-2, SLC
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Species
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Murine
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Accession
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P86792
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GeneID
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65956
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Source
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Escherichia coli.
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Molecular Weight
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重组小鼠exodus-2 / CCL21蛋白Approximately 12.1 kDa, a single, non-glycosylated polypeptide chain containing 110 amino acids.
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Quantity
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5µg/20µg/1000µg
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AA Sequence
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SDGGGQDCCL KYSQKKIPYS IVRGYRKQEP SLGCPIPAIL FLPRKHSKPE LCANPEEGWV QNLMRRLDQP PAPGKQSPGC RKNRGTSKSG KKGKGSKGCK RTEQTQPSRG
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Purity
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> 97 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组小鼠exodus-2 / CCL21蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using murine T-lymphocytes is in a concentration range of 10-100 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
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Endotoxin
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重组小鼠exodus-2 / CCL21蛋白Less than 1 EU/μg of rMuExodus-2/CCL21 as determined by LAL method.
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Reconstitution
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We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组小鼠exodus-2 / CCL21蛋白
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SDS-PAGE
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Reference
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1. Christopherson KW, 2nd, Campbell JJ, Hromas RA. 2001. Blood, 98: 3562-8.
2. Nguyen-Hoai T, Baldenhofer G, Sayed Ahmed MS, et al. 2012. Cancer Gene Ther, 19: 69-76.
3. Bonacchi A, Petrai I, Defranco RM, et al. 2003. Gastroenterology, 125: 1060-76.
4. Christopherson KW, 2nd, Hood AF, Travers JB, et al. 2003. Blood, 101: 801-6.
5. Banas B, Wornle M, Merkle M, et al. 2004. Kidney Int, 66: 2256-63.
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Background
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CCL21 is encoded by CCL21 gene that is located in Chr. 4 and shows 21-33 % identity to other CC chemokines. This chemokine contains the four conserved cysteines characteristic of β chemokines plus two additional cysteines in its unusually long carboxylterminal domain. Human and mouse CCL21 are highly conserved, exhibiting 86 % amino acid sequence identity. CCL21 is highly expressed in lymph nodes of certain endothelial cells, and in the spleen and appendix. It elicits effects by binding with CCR7, having functions of T and B lymphocytes chemotaxis and inhibiting hematopoiesis.
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