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- 产品名称：重组鼠单核细胞趋化蛋白-3 / CCL7蛋白
- 产品型号：rMuMCP-3/CCL7
- 产品展商：KALANG
- 产品文档：无相关文档
- 发布时间：2018-08-19
- 在线询价
简单介绍
重组鼠单核细胞趋化蛋白-3 / CCL7蛋白与其它公司提供的重组蛋白不同，rMuMCP-3/CCL7蛋白产品为采用CFS的无细胞麦胚蛋白合成系统表达出来的重组蛋白，可表达出对细胞有毒性、易被蛋白酶降解的蛋白；并获得具有良好的可溶性，并有翻译后修饰、从而部分具有功能的蛋白.同时独有的全自动蛋白纯化技术则简便高效，将蛋白纯化过程中对蛋白的损伤降低到*小程度.重组鼠单核细胞趋化蛋白-3 / CCL7蛋白（全长序列）产品可用于Western Blot验证、抗体制备、蛋白检测、ELISA等试验中.
产品描述
重组鼠单核细胞趋化蛋白-3 / CCL7蛋白
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Synonyms
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Protein FIC, Small-inducible Cytokine A7, Intercrine/chemokine MARC
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Species
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Murine
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Accession
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Q03366
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GeneID
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20306
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Source
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Escherichia coli.
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Molecular Weight
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重组鼠单核细胞趋化蛋白-3 / CCL7蛋白Approximately 8.5 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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QPDGPNASTC CYVKKQKIPK RNLKSYRRIT SSRCPWEAVI FKTKKGMEVC AEAHQKWVEE AIAYLDMKTP TPKP
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组鼠单核细胞趋化蛋白-3 / CCL7蛋白Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human monocytes is in a concentration range of 100-300 ng/ml.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in 2× PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/µg of rMuMCP-3/CCL7 as determined by LAL method.
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Reconstitution
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重组鼠单核细胞趋化蛋白-3 / CCL7蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组鼠单核细胞趋化蛋白-3 / CCL7蛋白
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SDS-PAGE
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Reference
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1. Kulmburg PA, Huber NE, Scheer BJ, et al. 1992. J Exp Med. 176:1773-8.
2. Thirion S, Nys G, Fiten P, et al. 1994. Biochem Biophys Res Commun. 201:493-9.
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Background
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Murine CCL7, also known as MARC, is belonging to the CC chemokine family. It is encoded by the gene CCL7 and was isolated from a mouse mast cell line after Fc epsilon RI triggering by IgE plus antigen. Sequence comparisons suggest that MARC may be the mouse homologue of the human MCP-3 gene. Similar to the human system, MARC/FIC is postulated the murine MCP-3. The MCP-3 protein family signals through CCR2 expect MCP-1 and possess cross-reacts across species. CCL7/MCP3 has chemotactic function for monocytes and eosinophils, but not for neutrophils.
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