|
Synonyms
|
T-cell Growth Factor, CGF
|
|
Species
|
Rat
|
|
Accession
|
P17108
|
|
GeneID
|
116562
|
|
Source
|
Escherichia coli.
|
|
Molecular Weight
|
重组大鼠白细胞介素-2蛋白Approximately 15.5 kDa, a single non-glycosylated polypeptide chain containing 135 amino acids.
|
|
Quantity
|
5µg/20µg/1000µg
|
|
AA Sequence
|
APTSSPAKET QQHLEQLLLD LQVLLRGIDN YKNLKLPMML TFKFYLPKQA TELKHLQCLE NELGALQRVL DLTQSKSFHL EDAGNFISNI RVTVVKLKGS ENKFECQFDD EPATVVEFLR RWIAICQSII STMTQ
|
|
Purity
|
> 97 % by SDS-PAGE and HPLC analyses.
|
|
Biological Activity
|
重组大鼠白细胞介素-2蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine CTLL-2 cells is less than 0.2 ng/ml, corresponding to a specific activity of > 5.0 × 106 IU/mg.
|
|
Physical Appearance
|
Sterile Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
A 0.2 μm filtered concentrated sterile solution in 10 mM sodium citrate, pH 4.0.
|
|
Endotoxin
|
Less than 1 EU/μg of rRtIL-2 as determined by LAL method.
|
|
Reconstitution
|
重组大鼠白细胞介素-2蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
|
|
Storage
|
This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
|
|
重组大鼠白细胞介素-2蛋白
|
|
|
SDS-PAGE
|
|
|
Reference
|
1. Ma, A., R. Koka, and P. Burkett. 2006. Annu Rev Immunol, 24: 657-79.
2. Taniguchi, T., H. Matsui, T. Fujita, et al. 1983. Nature, 302: 305-10.
3. Liparoto, S.F., D.G. Myszka, Z. Wu, et al. 2002. Biochemistry, 41: 2543-51.
4. Bodnar, A., E. Nizsaloczki, G. Mocsar, et al. 2008. Immunol Lett, 116: 117-25.
5. Mosmann, T.R., T. Yokota, R. Kastelein, et al. 1987. J Immunol, 138: 1813-6.
|
|
Background
|
IL-2 is a powerful immunoregulatory lymphokine produced by T-cells in response to antigenic or mitogenic stimulation. It is expressed by CD4+ and CD8+ T cells, γδ T cells, B cells, dendritic cells, and eosinophils. IL-2/IL-2R signaling is required for T-cell proliferation and other fundamental functions which are essential for the immune response. The receptor for IL-2 consists of three subunits (55 kDa IL2Rα, 75 kDa IL2Rβ, 64 kDa common gamma chain γc/IL2Rγ) that are present on the cell surface in varying preformed complexes. Recombinant rat IL-2 is a 15.3 kDa protein containing 134 amino acid residues and it shares about 82 %, 66 %, 66 %, 60 % and 54 % amino acid sequence identity with mouse, human, rhesus macaque, pig and equus caballus IL-2, respectively.
|
