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Species
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Rat
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Accession
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P56478
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GeneID
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25647
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Source
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Escherichia coli.
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Molecular Weight
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重组大鼠白细胞介素-7蛋白Approximately 14. 9 kDa, a single non-glycosylated polypeptide chain containing 129 amino acids.
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Quantity
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2µg/10µg/1000µg
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AA Sequence
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DCHIKDKDGK AFGSVLMISI NQLDKMTGTD SDCPNNEPNF FKKHLCDDTK EAAFLNRAAR KLRQFLKMNI SEEFNDHLLR VSDGTQTLVN CTSKEEKTIK EQKKNDPCFL KRLLREIKTC WNKILKGSI
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Purity
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> 95 % by SDS-PAGE and HPLC analyses.
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Biological Activity
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重组大鼠白细胞介素-7蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine 2E8 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg.
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Physical Appearance
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Sterile Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than 1 EU/µg of rRtIL-7 as determined by LAL method.
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Reconstitution
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重组大鼠白细胞介素-7蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
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Storage
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This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
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重组大鼠白细胞介素-7蛋白
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SDS-PAGE
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Reference
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1. Goodwin RG, Lupton S, Schmierer A, et al. 1989. Proc Natl Acad Sci U S A. 86:302-6.
2. Sutherland GR, Baker E, Fernandez KE, et al. 1989. Hum Genet. 82:371-2.
3. Lupton SD, Gimpel S, Jerzy R, et al. 1990. J Immunol. 144:3592-601.
4. Noguchi M, Nakamura Y, Russell SM, et al. 1993. Science. 262:1877-80.
5. Fry TJ, Mackall CL. 2002. Blood. 99:3892-904.
6. Fry TJ, Mackall CL. 2002. J Hematother Stem Cell Res. 11:803-7.
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Background
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Interleukin-7 (IL-7) is encoded by the IL7 gene and secreted by stromal cells in the red marrow and thymus. It binds to the IL-7 receptor, a heterodimer consisting of IL-7 receptor alpha and IL-2 receptor gamma chain. IL-7 stimulates the differentiation of hematopoietic stem cells into lymphoid progenitor cells and also stimulates proliferation of B cells, T cells and NK cells. Furthermore, IL-7 as an immunotherapy agent has been examined in many human clinical trials for various malignancies and during HIV infection. Rat IL-7 contains 129 amino acid residues and has three disulfide bonds. In addition, it has approximately 57 % and 88 % amino acid sequence identity with human and murine IL-7.
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